Interstitial cells of Cajal generate spontaneous transient depolarizations in the rat gastric fundus
Interstitial cells of Cajal generate spontaneous transient depolarizations in the rat gastric fundus
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DOI：
10.1152/ajpgi.00118.2009
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发表时间：
2009-10-01
影响因子：
4.5
通讯作者：
Suzuki, Hikaru
中科院分区：
文献类型：
--
作者：
Kito, Yoshihiko;Sanders, Kenton M.;Suzuki, Hikaru
Kito Y、Sanders KM、Ward SM、Suzuki H。Cajal 间质细胞在大鼠胃底产生自发短暂去极化。 Am J Physiol Gastrointest Liver Physiol 297：G814-G824，2009。首次发表于 2009 年 7 月 30 日； doi: 10.1152/ajpgi.00118.2009.-细胞内记录是由大鼠胃底的分离的圆形肌束进行的。大多数细胞产生持续的电活动放电（持续 15 分钟），导致染料在 CSMC 之间、ICC-IM 之间以及 CSMC 和 ICC-IM 之间扩散。观察到两种类型的 STD：有规律发生的连续性 STD 和不规则的噪声突发性 STD。两类 STD 之间的 STD 发病率有所不同。单个单位加起来可产生最大幅度 30 mV 的 STD。硝普钠 (3 μM) 诱导膜超极化并消除 CSMC 产生的单一电位。相反，ICC-IM 产生的 STD 幅度随着膜超极化而增加。吡那地尔 (10 μM) 诱导的超极化也增加了 STD 的幅度并增强了 dV/dt(max)。这些观察结果表明，ICC-IM 中产生的 STD 被动传播到邻近的 CSMC，从而引起胃底单一电位的放电。
Kito Y, Sanders KM, Ward SM, Suzuki H. Interstitial cells of Cajal generate spontaneous transient depolarizations in the rat gastric fundus. Am J Physiol Gastrointest Liver Physiol 297: G814-G824, 2009. First published July 30, 2009; doi: 10.1152/ajpgi.00118.2009.-Intracellular recordings were made from isolated circular muscle bundles of rat gastric fundus. The majority of cells generated an ongoing discharge of electrical activity that were 15 min) resulted in the spread of dye between CSMC, between ICC-IM, and between CSMC and ICC-IM. Two types of STDs were observed, regularly occurring continuous STDs and irregular noisy bursting STDs. The amplitude of STDs varied between the two types of STDs. Single units summed to develop STDs with a maximum amplitude of 30 mV. Sodium nitroprusside (3 mu M) induced membrane hyperpolarization and abolished unitary potentials generated by CSMC. In contrast, the amplitude of STDs generated by ICC-IM was increased with membrane hyperpolarization. Hyperpolarization induced by pinacidil (10 mu M) also increased the amplitude of STDs and enhanced dV/dt(max). These observations indicate that STDs generated in ICC-IM spread passively to the adjacent CSMC to evoke the discharge of unitary potentials in the gastric fundus.