CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4.
CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4.
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DOI：
10.1186/s11671-016-1355-3
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发表时间：
2016-12
影响因子：
--
通讯作者：
He J
中科院分区：
文献类型：
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作者：
Yan X;Wang K;Lu W;Qin W;Cui D;He J
碳水化合物抗原72-4（CA72-4）是与早期胃癌的诊断和预后密切相关的重要生物标志物。如何实现临床标本中CA72-4的快速、灵敏、特异、定量检测已成为一个很大的要求。在此，我们报道了一种 CdSe/ZnS 量子点标记的横向流动测试条与基于电荷耦合器件 (CCD) 的读取器相结合，用于快速、灵敏和定量检测 CA72-4。使用两种针对 CA72-4 的小鼠单克隆抗体 (mAb)。其中一个被涂覆作为测试线，而另一个 mAb 用量子点标记并涂覆到结合垫上。固定山羊抗小鼠 IgG 作为对照线。添加样品后，CA72-4 和这两种 mAb 形成三明治结构。夹心结构中量子点 (QD) 标记的 mAb 的荧光信号与检测到的 CA72-4 的量相关。使用基于CCD的读数器实现CA72-4的定量检测。结果表明，开发的 QD 标记侧流试纸条可检测 CA72-4 生物标志物，灵敏度为 2 IU/mL，检测时间为 10 分钟。使用来自临床胃癌患者和健康人的一百份血清样本来证实该试纸条法的特异性；结果表明，所建立的条带法与罗氏电化学发光检测结果相比，具有100%的重现性和100%的特异性。总之，用于检测CA72-4的CdSe/ZnS量子点标记侧流层析试纸可以实现临床样本的快速、灵敏、特异检测，在不久的将来在临床转化方面具有巨大的潜力。
Carbohydrate antigen 72-4 (CA72-4) is an important biomarker associated closely with diagnosis and prognosis of early gastric cancer. How to realize quick, sensitive, specific, and quantitative detection of CA72-4 in clinical specimens has become a great requirement. Herein, we reported a CdSe/ZnS quantum dot-labeled lateral flow test strip combined with a charge-coupled device (CCD)-based reader was developed for rapid, sensitive, and quantitative detection of CA72-4. Two mouse monoclonal antibodies (mAbs) against CA72-4 were employed. One of them was coated as a test line, while another mAb was labeled with quantum dots and coated onto conjugate pad. The goat anti-mouse IgG was immobilized as a control line. After sample was added, a sandwich structure was formed with CA72-4 and these two mAbs. The fluorescent signal from quantum dots (QD)-labeled mAb in sandwich structure was related to the amount of detected CA72-4. A CCD-based reader was used to realize quantitative detection of CA72-4. Results showed that developed QD-labeled lateral flow strips to detect CA72-4 biomarker with the sensitivity of 2 IU/mL and 10 min detection time. One hundred sera samples from clinical patients with gastric cancer and healthy people were used to confirm specificity of this strip method; results showed that established strip method own 100 % reproducibility and 100 % specificity compared with Roche electrochemiluminescence assay results. In conclusion, CdSe/ZnS quantum dot-labeled lateral flow strips for detection of CA72-4 could realize rapid, sensitive, and specific detection of clinical samples and could own great potential in clinical translation in near future.