Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti
Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti
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DOI：
10.1073/pnas.95.7.3748
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发表时间：
1998-03-31
影响因子：
11.1
通讯作者：
James, AA
中科院分区：
文献类型：
--
作者：
Coates, CJ;Jasinskiene, N;James, AA
水手转座因子能够在黄热病蚊子，埃及伊蚊的胚胎体细胞中进行质粒间转座。为了确定这种表现出的移动性是否可以用于对蚊子进行遗传改造，将一种带有黑胃果蝇朱砂基因野生型等位基因的改良水手元件微注射到犬尿氨酸羟酶缺乏的白眼受体菌株的胚胎中。微注射胚胎产生的69个可生育的雄性创始人中有3个产生了具有有色眼睛的后代个体，转化率为4%。转基因介导的eve color互补被观察到以孟德尔方式分离，尽管一个插入与性别决定位点的隐性等位基因（雌性决定）分离，并且单独的插入是纯合致死的。对选定的转化家族的分子分析表明，在每种情况下，该结构的单个完整拷贝都以转座酶介导的方式独立整合。水手转化系统的可用性大大提高了我们研究和操纵这一重要媒介物种的能力。
The mariner transposable element is capable of interplasmid transposition in the embryonic soma of the yellow fever mosquito, Aedes aegypti. To determine if this demonstrated mobility could be utilized to genetically transform the mosquito, a modified mariner element marked with a wild type allele of the Drosophila melanogaster cinnabar gene was microinjected into embryos of a kynurenine hydroxylase-deficient, white-eyed recipient strain. Three of 69 fertile male founders resulting from the microinjected embryos produced families with colored-eyed progeny individuals, a transformation rate of 4%. The transgene-mediated complementation of eve color was observed to segregate in a Mendelian manner, although one insertion segregates with the recessive allele (female-determining) of the sex-determining locus, and a separate insertion is homozygous lethal. Molecular analysis of selected transformed families demonstrated that a single complete copy of the construct had integrated independently in each case acid that it had done so in a transposase-mediated manner. The availability of a mariner transformation system greatly enhances our ability to study and manipulate this important vector species.