Identification of major walnut cultivars grown in China based on nut phenotypes and SSR markers
Identification of major walnut cultivars grown in China based on nut phenotypes and SSR markers
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DOI：
10.1016/j.scienta.2014.02.004
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发表时间：
2014-03-26
影响因子：
4.3
通讯作者：
Pei, Dong
中科院分区：
文献类型：
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作者：
Chen, LingNa;Ma, QingGuo;Pei, Dong
核桃（Juglans regia L）是一种重要的经济树种，因其坚果和木材而广泛种植于温带地区。本研究利用12个SSR （simple sequence repeat）标记和22个坚果形状性状对中国35个核桃品种进行了分子鉴定。根据果实表型将35个品种划分为11大类。纵切面到缝线的形状特征为宽椭圆形或以椭圆形为最大类别，共有18个品种。每个品类的所有材料都被不超过2对SSR引物区分。除了坚果表型外，利用4对引物还能鉴定出35个品种，每个品种都有独特的指纹图谱。特别是，具有相同亲本的品种，如辽宁1号和辽宁3号，由于坚果形状不同，很容易分化。无论坚果表型性状如何，只要4个位点（WJR031、WJR069、WJR265和WJR281）就可以对35个品种进行明确的分离。该研究为核桃品种的鉴定、鉴定和鉴定提供了一个有用的方案，也显示了SSR标记在品种鉴定和知识产权保护中的作用。此外，核桃形状分类对核桃批量加工和区域品种选择具有重要意义，可用于核桃工业。(C) 2014 Elsevier B.V.版权所有
Walnut (Juglans regia L) is an economically important tree widely cultivated in temperate regions for its nuts and timber. In this study, molecular identification using 12 simple sequence repeat (SSR) markers and 22 nut-shape characters were conducted on 35 cultivars of walnut grown in China. All 35 cultivars were classified into 11 broad categories on the basis of the nut phenotype. The shape characteristic of longitudinal sections to the suture presented a broad elliptic or elliptic shape was the largest category and included 18 cultivars. All accessions in each category were differentiated by no more than two SSR primer pairs. In addition to nut phenotype, 35 cultivars could be identified using four primer pairs, and every cultivar presented unique fingerprints. In particular, cultivars with the same parents were easily differentiated, such as 'Liaoning 1' and 'Liaoning 3' based on their different nut shape. Regardless of nut phenotype characters, just four loci (WJR031, WJR069, WJR265, and WJR281) also allowed the unambiguous separation of the 35 cultivars studied. This study provides a useful protocol for the characterization, identification, and authentication of any walnut cultivar and also shows the power of SSR markers in cultivar identification and intellectual property protection. Furthermore, the classification of nut shape is important for nut batch processing and for regional cultivar selection, which could be useful for the walnut industry. (C) 2014 Elsevier B.V. All rights reserved.