Analysis of gut microbiota and metabolites in patients with rheumatoid arthritis and identification of potential biomarkers.
Analysis of gut microbiota and metabolites in patients with rheumatoid arthritis and identification of potential biomarkers.
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DOI：
10.18632/aging.203641
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发表时间：
2021-10-20
期刊：
影响因子：
--
通讯作者：
Dai Y
中科院分区：
文献类型：
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作者：
Chen Y;Ma C;Liu L;He J;Zhu C;Zheng F;Dai W;Hong X;Liu D;Tang D;Dai Y
类风湿性关节炎（RA）是一种自身免疫性疾病，主要表现为关节破坏、滑膜炎和血管翳形成.肠道微生物群作为环境因素在RA中起着重要作用，但关于RA中微生物组的致病机制的研究很少。我们使用16 S rRNA基因测序和基于超高效液相色谱-质谱的代谢组学的综合方法来分析RA患者与健康受试者相比的肠道植物群和肠道微生物群代谢物的结构和多样性。在这项研究中，肠道微生物群的α多样性分析表明，健康对照组（HC）和RA组之间没有显著差异。但β-多样性分析显示两组间差异有统计学意义。对肠道微生物群变化的进一步分析表明，在门水平上，RA组中p_拟杆菌的相对丰度显著降低，而RA组中疣微菌属和变形菌属的相对丰度显著增加。在属水平上，类杆菌属、粪杆菌属和部分益生菌在RA组中减少，而乳杆菌属、链球菌属和阿克曼氏菌属等97个属在RA组中增加。在HC和RA组之间鉴定了74种丰度差异的代谢物，我们在RA中鉴定了两种潜在的生物标志物（9，12-十八碳二炔酸和10 Z-十九碳烯酸）。
Rheumatoid arthritis (RA) is an autoimmune disease described by joint destruction, synovitis and pannus formation. The gut microbiota acts as an environmental factor that plays an important role in RA, but little research regarding the etiopathogenic mechanisms of the microbiome in RA has been carried out. We used an integrated approach of 16S rRNA gene sequencing and ultrahigh-performance liquid chromatography-mass spectrometry-based metabolomics to analyze the structure and diversity of the intestinal flora and metabolites of the gut microbiota in RA patients compared with healthy subjects. In this study, α-diversity analysis of the gut microbiota showed that there was no significant difference between the healthy control (HC) and RA groups. However, β-diversity analysis showed that there was a significant difference between the two groups. Further analysis of alteration of the gut microbiota revealed that at the phylum level, the relative abundance of p_Bacteroidetes was significantly decreased in the RA group, while that of Verrucomicrobia and Proteobacteria was significantly increased in the RA group. At the genus level, Bacteroides, Faecalibacterium and some probiotics were decreased in the RA group, while 97 genera, including Lactobacillus, Streptococcus and Akkermansia, were increased in the RA group. Seventy-four differentially abundant metabolites were identified between the HC and RA groups, and we identified two potential biomarkers (9,12-octadecadiynoic acid and 10Z-nonadecenoic acid) in RA.