Swollenin aids in the amorphogenesis step during the enzymatic hydrolysis of pretreated biomass
Swollenin aids in the amorphogenesis step during the enzymatic hydrolysis of pretreated biomass
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DOI：
10.1016/j.biortech.2013.05.053
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发表时间：
2013-08-01
影响因子：
11.4
通讯作者：
Saddler, Jack
中科院分区：
文献类型：
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作者：
Gourlay, Keith;Hu, Jinguang;Saddler, Jack
整个水解过程中的一个关键限制是由于纤维素的宏观和微观结构及其与半纤维素和木质素的结合，水解酶的进入受到限制。先前的工作表明，当使用更纯形式的纤维素时，几种非水解蛋白质可以破坏纤维素结构并提高水解酶的活性。在这里报道的工作中，膨胀素主要破坏预处理玉米秸秆的半纤维素部分，导致单糖和低聚糖的溶解。尽管膨胀素在与纤维素酶单组分外切葡聚糖酶（CEL7A）和内切葡聚糖酶（CEL5A）组合时显示出很小的协同作用，但它与木聚糖酶单组分木聚糖酶GH10和木聚糖酶GH11显示出显著的协同作用，导致显著更多的木糖（>300%）的释放。似乎膨胀素在无定形发生中起作用，并且其主要作用是增强对半纤维素级分的接近，所述半纤维素级分限制或掩盖对木质纤维素底物的纤维素组分的接近。(C)2013作者由爱思唯尔有限公司出版。保留所有权利。
A key limitation in the overall hydrolysis process is the restricted access that the hydrolytic enzymes have due to the macro-and-micro structure of cellulose and its association with hemicellulose and lignin. Previous work has shown that several non-hydrolytic proteins can disrupt cellulose structure and boost the activity of hydrolytic enzymes when purer forms of cellulose are used. In the work reported here, Swollenin primarily disrupted the hemicellulosic fraction of pretreated corn stover, resulting in the solubilisation of monomeric and oligomeric sugars. Although Swollenin showed little synergism when combined with the cellulase monocomponents exoglucanase (CEL7A) and endoglucanase (CEL5A), it showed pronounced synergism with xylanase monocomponents Xylanase GH10 and Xylanase GH11, resulting in the release of significantly more xylose (>300%). It appears that Swollenin plays a role in amorphogenesis and that its primary action is enhancing access to the hemicellulose fraction that limits or masks accessibility to the cellulose component of lignocellulosic substrates. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.