XANTHINE-OXIDASE DEHYDROGENASE IN MAMMARY-GLAND OF MOUSE - RELATIONSHIP TO MAMMOGENESIS AND LACTOGENESIS INVIVO AND INVITRO
XANTHINE-OXIDASE DEHYDROGENASE IN MAMMARY-GLAND OF MOUSE - RELATIONSHIP TO MAMMOGENESIS AND LACTOGENESIS INVIVO AND INVITRO
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DOI：
10.1017/s0022029900030004
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发表时间：
1991-11-01
影响因子：
2.1
通讯作者：
MURPHY, P
中科院分区：
文献类型：
--
作者：
HAYDEN, TJ;BRENNAN, D;MURPHY, P
黄嘌呤氧化酶/脱氢酶（XO/XDH）在妊娠中期小鼠乳腺中升高，但在肝脏中不升高，并一直升高到产后20 d断奶。酶活性的增加既不是由于激活剂或抑制剂的改变，也不是由于产生了具有改变的催化性质的变体酶。在体内增加之前，血浆中催乳素样活性（胎盘催乳素）激增，并且催乳素是体外外植体培养中诱导XO/XDH所需的。XO/XDH在体内和体外的诱导先于腺体的完全组织学分化。此外，XO/XDH在体外诱导发生更迅速，催乳素浓度较低，比组织学分化。因此，尽管XO/XDH存在于乳汁中，但XO/XDH活性增加是体内和体外乳房发生的早期事件，而不是分化的终末组分。
Xanthine oxidase/dehydrogenase (XO/XDH) increases at mid gestation in mammary gland but not in liver of the mouse and remains elevated until the pups are weaned at 20 d post partum. The increase in enzyme activity is due neither to alteration in activators or inhibitors nor to a production of a variant enzyme with altered catalytic properties. The increase is preceded in vivo by a surge of prolactin-like activity (placental lactogen) in plasma, and prolactin is required for induction of XO/XDH in explant culture in vitro. Induction of XO/XDH in vivo and in vitro precedes the full histological differentiation of the gland. In addition, induction of XO/XDH in vitro occurs more rapidly and at lower concentrations of prolactin than does histological differentiation. Thus although XO/XDH is present in milk, increased XO/XDH activity is an early event in mammogenesis in vivo and in vitro rather than a terminal component of differentiation.