Distribution of BMP6 in the alveolar bone during mouse mandibular molar eruption
Distribution of BMP6 in the alveolar bone during mouse mandibular molar eruption
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DOI：
10.3109/03008207.2014.951441
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发表时间：
2014-08
影响因子：
2.9
通讯作者：
V. Oralová;I. Chlastáková;R. Radlanski;E. Matalova
中科院分区：
文献类型：
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作者：
V. Oralová;I. Chlastáková;R. Radlanski;E. Matalova
摘要萌出需要牙齿与周围组织，特别是骨骼的同步。萌出过程中的一个重要步骤是牙齿基部和沿着牙根的牙槽骨重塑。据报道，BMP 6的表达在萌出前牙囊的基底半部增加，并且抑制BMP 6影响牙槽窝基部的骨形成。本研究的目的是进一步研究骨形成蛋白6蛋白与牙齿萌出和相应的骨重建使用的时空相关性的骨形成蛋白6的定位与形态发生事件（增殖，分化，凋亡和骨沉积/吸收），其他骨形成蛋白（骨形成蛋白2和骨形成蛋白7）和三维图像的牙齿-骨发育。BMP 6在下颌磨牙及萌出周围相关结构中的表达模式。BMP 6的定位主要在成骨细胞中，在牙槽窝内的骨形成区域中。这些发现与牙根区域的增殖、成骨细胞/骨细胞中骨钙素的表达以及牙齿周围牙槽骨中BMP 2和BMP 7的存在正相关。破骨细胞活性和凋亡消除在根区逐渐下降，在喷发前和喷发阶段完全停止。通常，BMP 6与BMP 2、BMP 7和骨钙素阳性成骨细胞以及骨重建区域呈正相关。此外，BMP 6在牙周组织和成牙骨质细胞中也有表达。BMP 6在牙槽骨中的表达与牙萌出有关。值得注意的是，在同一发育阶段，骨中BMP 6的表达模式在单个臼齿周围没有差异。BMP 6在牙周韧带中的表达可能参与了萌出和锚定过程中牙齿与骨之间的相互作用。
Abstract Eruption requires synchrony of the tooth with the surrounding tissues, particularly the bone. One important step during eruption is remodelling of the alveolar bone at the base of the tooth and along the roots. Expression of BMP6 was reported to be increased in the basal half of the dental follicle prior to eruption and inhibition of BMP6 affected bone formation at the base of the alveolar crypt. The aim of this study was to further investigate BMP6 protein in relation to tooth eruption and the corresponding bone remodelling using temporospatial correlations of BMP6 localization with morphogenetic events (proliferation, differentiation, apoptosis and bone apposition/resorption), other BMPs (BMP2 and BMP7) and three-dimensional images of tooth–bone development. BMP6 expression pattern was mapped in the mandibular molar teeth and related structures around eruption. Localization of BMP6 dominated in osteoblasts, in regions of bone formation within the alveolar crypt. These findings positively correlated with proliferation at the tooth base region, osteocalcin expression in the osteoblasts/osteocytes and BMP2 and BMP7 presence in the alveolar bone surrounding the tooth. Osteoclast activity and apoptotic elimination in the root region gradually decreased before eruption and totally ceased at eruption stages. Generally, BMP6 positively correlated with BMP2, BMP7 and osteocalcin-positive osteoblasts, and areas of bone remodelling. Moreover, BMP6 was found in the periodontium and cementoblasts. BMP6 expression in the alveolar bone accompanied tooth eruption. Notably, the expression pattern of BMP6 in the bone did not differ around individual molar teeth at the same stage of development. The expression of BMP6 in periodontal ligaments may contribute to interaction between the tooth and bone during the eruption and anchoring process.