Dusky works upstream of Four-jointed and Forked in wing morphogenesis in Tribolium castaneum
Dusky works upstream of Four-jointed and Forked in wing morphogenesis in Tribolium castaneum
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DOI：
10.1111/imb.12327
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发表时间：
2017
影响因子：
2.6
通讯作者：
Chen K.
中科院分区：
文献类型：
--
作者：
Li C.;Li B.;Ma S.;Lue P.;Chen K.
在黑腹果蝇的翅膀形态发生过程中，Dusky（dy）是细胞骨架重组所必需的，但哪些基因参与了翅膀形态发生尚不清楚。tribolium castaneum，dyis在胚胎后期高度表达。组织特异性表达分析表明，后期幼虫的表皮、头部和脂肪体中dyy的表达水平较高。RNA干扰（RNAi）显著降低了成虫的翅膀大小，并导致鞘翅折叠不当。同时，dyknockdown减少了four - jointed（fj）和forked(f)的转录。我们的研究结果表明，fjrnai减少了成虫翅膀的大小，沉默导致了异常的翅膀折叠。castaneum。有趣的是，同时敲除jj和表型也会影响rnai，这表明rnai可能在上游起作用，调节翅膀的形态发生。castaneum。
Dusky(dy) is required for cytoskeletal reorganization during wing morphogenesis inDrosophila melanogaster, but which genes participate together withdyfor wing morphogenesis has remained unclear. InTribolium castaneum,dyis highly expressed at the late embryonic stage. Tissue‐specific expression analysis indicated high expression levels ofdyin the epidermis, head and fat body of late‐stage larvae. RNA interference (RNAi) targetingdysignificantly decreased adult wing size and caused improper folding of the elytra. Meanwhile,dyknockdown reduced the transcription offour‐jointed(fj) andforked(f). Our results show thatfjRNAi reduces adult wing size and that silencingfresults in abnormal wing folding inT. castaneum. Interestingly, knocking downfjandfsimultaneously phenocopiesdyRNAi, suggesting thatdyprobably acts upstream offjandfto regulate wing morphogenesis inT. castaneum.