Functional correlates of activity in neurons projecting from the lamina terminalis to the ventrolateral periaqueductal gray.
Functional correlates of activity in neurons projecting from the lamina terminalis to the ventrolateral periaqueductal gray.
复制标题
从终板投射到腹外侧导水管周围灰质的神经元活动的功能相关性。
DOI：
10.1111/j.1460-9568.2009.07024.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
McKinley,MichaelJ
中科院分区：
文献类型：
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作者：
Uschakov,Aaron;McGinty,Dennis;Szymusiak,Ronald;McKinley,MichaelJ
终末层（LT）由LT血管器官（OVLT）、正中视前核（MnPO）和皮质下器官（SFO）组成。LT的所有细分都指向腹外侧导水管周围灰质（vlPAG）。LT和vlPAG涉及多种内稳态和行为功能，包括体液内稳态、体温调节和睡眠和清醒调节。通过结合c‐Fos蛋白可视化和逆行神经解剖示踪剂，我们研究了LT‐vlPAG投射神经元的功能相关性。将逆行示踪剂注射到大鼠的vlPAG中，经过1周的恢复期后，它们分别接受高渗盐水（0.5mNaCl, 1 mL/100 g/ p）、24小时缺水、异丙肾上腺素（增加循环血管紧张素II； 50 μg/kg s.c）、热暴露（39°C 60分钟）或允许180分钟的自然睡眠。然后在整个实验过程中对vlPAG逆行标记的神经元和双标记的神经元进行鉴定和量化。OVLT - vlPAG投射神经元对高渗盐水和水剥夺反应最明显。SFO‐vlPAG投射神经元在异丙肾上腺素处理后最为活跃，而MnPO‐vlPAG投射神经元在缺水、热暴露和睡眠后显示出更多的Fos免疫染色。这些结果支持了LT - vlPAG -投射神经元功能细分的存在，并指出了三种活动模式，分别对应于热和睡眠觉醒调节、渗透或激素刺激。
The lamina terminalis (LT) consists of the organum vasculosum of the LT (OVLT), the median preoptic nucleus (MnPO) and the subfornical organ (SFO). All subdivisions of the LT project to the ventrolateral periaqueductal gray (vlPAG). The LT and the vlPAG are implicated in several homeostatic and behavioral functions, including body fluid homeostasis, thermoregulation and the regulation of sleep and waking. By combining visualization of c‐Fos protein and retrograde neuroanatomical tracer we have examined the functional correlates of LT‐vlPAG projection neurons. Rats were injected with retrograde tracer into the vlPAG and, following a 1‐week recovery period, they were subjected to either hypertonic saline administration (0.5mNaCl, 1 mL/100 g i.p.), 24‐h water deprivation, isoproterenol administration (increases circulating angiotensin II; 50 μg/kg s.c.), heat exposure (39°C for 60 min) or permitted 180 min spontaneous sleep. Retrogradely labeled neurons from the vlPAG and double‐labelled neurons were then identified and quantified throughout the LT. OVLT‐vlPAG projection neurons were most responsive to hypertonic saline and water deprivation. SFO‐vlPAG projection neurons were most active following isoproterenol administration, and MnPO‐vlPAG projection neurons displayed significantly more Fos immunostaining following water deprivation, heat exposure and sleep. These results support the existence of functional subdivisions of LT‐vlPAG‐projecting neurons, and indicate three patterns of activity that correspond to thermal and sleep wake regulation, osmotic or hormonal stimuli.