Streptomycetes in micro-cultures: Growth, production of secondary metabolites, and storage and retrieval in the 96-well format
Streptomycetes in micro-cultures: Growth, production of secondary metabolites, and storage and retrieval in the 96-well format
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DOI：
10.1023/a:1010254013352
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发表时间：
2000-12-01
影响因子：
2.6
通讯作者：
Duetz, W
中科院分区：
文献类型：
--
作者：
Minas, W;Bailey, JE;Duetz, W
形成菌丝体的链霉菌菌株在方形深孔微量滴定板中的一毫升液体微培养物中生长。根据生物量形成和次生代谢物的产生来评估生长，发现它们分别在微培养物、生物反应器和摇瓶培养中非常相似。尽管进行了重复采样并且孔壁上广泛生长，但没有发生交叉污染。此外，我们成功地将孢子悬浮液（96 孔格式）冷藏在 -20 摄氏度下，直接从微量滴定板琼脂上生长的培养物制备。通过从冷冻孢子悬浮液中复制等分试样来回收培养物。
Mycelium-forming Streptomyces strains were grown in one milliliter liquid micro-cultures in square deep-well microtiter plates. Growth was evaluated with respect to biomass formation and production of secondary metabolites which were found to be very similar in the micro-cultures, bioreactor, and shake flask cultivations, respectively. Despite repetitive sampling and extensive growth on the walls of the wells, no cross contamination occurred. Furthermore, we successfully employed cold storage at -20 degreesC of spore suspensions (in the 96-well format), directly prepared from cultures grown on agar in the microtitre plate. Cultures were retrieved by replicating aliquots from the frozen spore suspensions.