Hydrogen gas distribution in organs after inhalation: Real-time monitoring of tissue hydrogen concentration in rat
Hydrogen gas distribution in organs after inhalation: Real-time monitoring of tissue hydrogen concentration in rat
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DOI：
10.1038/s41598-018-38180-4
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发表时间：
2019-02-04
影响因子：
4.6
通讯作者：
Sasaki, Junichi
中科院分区：
文献类型：
--
作者：
Yamamoto, Ryo;Homma, Koichiro;Sasaki, Junichi
氢对各种疾病有治疗和预防作用。尽管动物和临床研究报告了有希望的结果，但氢在给药后器官中的分布仍不清楚。在这里，使用高灵敏度的玻璃微传感器和连续吸入3%的氢气来监测组织中氢浓度随时间的顺序变化。测定大鼠脑、肝、肾、肠系膜脂肪和大腿肌肉中的氢浓度。从浓度曲线中得到了最大浓度、饱和时间和其他代表分布动态的测量值，并对不同器官的结果进行了比较。达到饱和的时间明显更长（20.2 vs 6.3-9.4分钟）。P = 0.004)，肌肉比其他脏器逐渐升高。最大浓度以肝脏最高，肾脏最低（29.0 +/- 2.6 vs 18.0 +/- 2.2 μ mol/L, P = 0.03）。不同脏器浓度差异显著（P = 0.03）。这些结果为阐明氢气在哺乳动物体内有利作用的机制提供了基础。
Hydrogen has therapeutic and preventive effects against various diseases. Although animal and clinical studies have reported promising results, hydrogen distribution in organs after administration remains unclear. Herein, the sequential changes in hydrogen concentration in tissues over time were monitored using a highly sensitive glass microsensor and continuous inhalation of 3% hydrogen gas. The hydrogen concentration was measured in the brain, liver, kidney, mesentery fat and thigh muscle of rats. The maximum concentration, time to saturation, and other measurements representing the dynamics of distribution were obtained from the concentration curves, and the results obtained for different organs were compared. The time to saturation was significantly longer (20.2 vs 6.3-9.4 min. P = 0.004 in all cases) and increased more gradually in muscle than in the other organs. The maximum concentration was the highest in liver and the lowest in the kidney (29.0 +/- 2.6 vs 18.0 +/- 2.2 mu mol/L; P = 0.03 in all cases). The concentration varied significantly depending on the organ (P = 0.03). These results provide the fundamentals for elucidating the mechanisms underlying the in vivo favourable effects of hydrogen gas in mammalian systems.