Hsp70 expression in human skeletal muscle after exercise.
Hsp70 expression in human skeletal muscle after exercise.
复制标题
运动后人体骨骼肌中 Hsp70 的表达。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Rudolf Billeter
中科院分区：
文献类型：
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作者：
A. Puntschart;Michael Vogt;Hans R. Widmer;Hans Hoppeler;Rudolf Billeter
足够高强度的长时间运动被认为会产生生理压力，扰乱细胞的动态平衡，最终诱导细胞适应，使机体能够更好地应对未来的运动挑战。热休克蛋白(HSP)在细胞暴露于不同类型的应激时表达。在这项研究中，我们研究了热诱导形式的HSP70在人类骨骼肌细胞中的表达是否在单次运动后增加。五名未经训练的受试者在跑步机上以各自的无氧阈值进行了30分钟的锻炼。运动后4min，HSP70mRNA浓度显著升高4倍。在运动结束后30分钟和3小时也观察到类似的高水平。HSP70蛋白浓度在运动停止后3h内无明显变化。因此，人类的一次运动能够增加HSP70 mRNA的稳态浓度，但可能不足以对其已经很高的基础蛋白质水平产生影响。HSP70mRNA的分析作为一种检测热休克蛋白基础水平较高的组织中的应激反应的方法具有潜在的实用价值。
Prolonged exercise of a sufficiently high intensity is thought to create physiological stress and to disturb cellular homeostasis, ultimately inducing cellular adaptations which enable the organism to better deal with any future exercise challenge. Heat shock proteins (hsp) are expressed when cells are exposed to different types of stress. In this study, we have investigated whether the expression of the heat inducible form of hsp70 is increased in human skeletal muscle cells after a single bout of exercise. Five untrained subjects performed an exercise bout at their individual anaerobic threshold for 30 min on a treadmill. Hsp70 mRNA concentration was significantly increased by a factor of four at 4 min post-exercise. Similarly high levels were also observed 30 min and 3 h after the end of exercise. Hsp70 protein concentration, on the contrary, did not change within 3 h after cessation of exercise. Thus, a single exercise bout in humans is able to increase the steady state concentration of hsp70 mRNA, but is probably not sufficient to have an effect on the already high basal level of its protein. The analysis of hsp70 mRNA is potentially useful as a method to detect stress in tissues with a high basal level of heat shock proteins.