Bioelectric effect of a chitosan bioelectret membrane on bone regeneration in rabbit cranial defects
Bioelectric effect of a chitosan bioelectret membrane on bone regeneration in rabbit cranial defects
复制标题
DOI：
10.1177/0883911512436773
复制
发表时间：
2012-03
影响因子：
1.7
通讯作者：
Yanying Wang;Rui Shi;P. Gong;Jidong Li;Juan Li;Danting Ao;Ping Wang;Yang Yang-Yang;Y. Man;Y. Qu
中科院分区：
文献类型：
--
作者：
Yanying Wang;Rui Shi;P. Gong;Jidong Li;Juan Li;Danting Ao;Ping Wang;Yang Yang-Yang;Y. Man;Y. Qu
在这项研究中，壳聚糖生物驻极体膜对骨再生的兔颅骨缺损模型的影响进行了检查。生物驻极体通过薄膜浇铸制造，并通过栅控电晕充电（-1 kV）极化。在体外实验中，生物驻极体的等效表面电荷密度在潮湿和干燥条件下逐渐降低。在体实验中，在兔颅骨上对称地制作2个8 mm × 10 mm的矩形骨缺损。分别用生物驻极体膜、非极化膜和无膜处理缺损。分别于术后4、8、12周处死动物，行显微CT和组织学检查。生物驻极体膜受体有一个显着更高的体积新形成的骨和更大的速度比非极化膜的材料降解。此外，生物驻极体膜不仅在宿主骨周围而且在缺损中心诱导新骨形成。壳聚糖生物驻极体膜在引导性骨再生中的应用具有明显的潜力。
In this study, the effects of a chitosan bioelectret membrane on bone regeneration in a rabbit cranial defect model were examined. The bioelectret was fabricated by film casting and polarized by grid-controlled corona charging (−1 kV). In vitro, the equivalence surface charge density of the bioelectret gradually reduced under both wet and dry conditions. In vivo, two rectangular transosseous defects of 8 mm × 10 mm were created symmetrically on each rabbit cranial bone. The defects were treated with the bioelectret membrane, with unpolarized membrane, and no membrane, respectively. The rabbits were killed at 4, 8, and 12 weeks postoperatively, and specimens were examined by micro-computed tomography and histological analysis. The bioelectret membrane recipients had a significantly higher volume of newly formed bone and greater rate of material degradation than the unpolarized membrane. Furthermore, the bioelectret membrane induced new bone formation not only around the host bone but also in the center of the defects. The chitosan bioelectret membrane application has an apparent potential in guided bone regeneration applications.