Midget and parasol ganglion cells of the primate retina express the α1 subunit of the glycine receptor
Midget and parasol ganglion cells of the primate retina express the α1 subunit of the glycine receptor
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DOI：
10.1017/s0952523899165155
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发表时间：
1999-09-01
影响因子：
1.9
通讯作者：
Ghosh, KK
中科院分区：
文献类型：
--
作者：
Grünert, U;Ghosh, KK
甘氨酸是哺乳动物视网膜中一种主要的抑制性神经递质，已被证明能影响神经节细胞的反应。灵长类动物视网膜的小神经节细胞和伞神经节细胞具有不同的生理作用，对光刺激的反应特征也存在差异。在本研究中，我们探讨了甘氨酸受体在侏儒神经节细胞和伞神经节细胞中的表达是否不同。将神经生物素注射到狨猴和猕猴视网膜神经节细胞中，制备活体视网膜全安装体。然后用针对甘氨酸受体α 1亚基的抗体处理视网膜碎片。内丛状层的ON和OFF膜下存在强烈的点状免疫反应性，表明突触定位。许多免疫反应点与侏儒神经节和伞神经节细胞的树突相吻合。免疫反应点存在于on和OFF细胞的远端和近端树突上。这些结果表明，在甘氨酸受体α 1亚基的分布方面，ON和OFF小细胞和伞细胞没有差异。
Glycine is a major inhibitory neurotransmitter in the mammalian retina and has been shown to influence the responses of ganglion cells. Midget and parasol ganglion cells serve distinct physiological roles in the primate retina and show differences in their response characteristics to light stimuli. In the present study, we addressed the question of whether the expression of glycine receptors differs in midget and parasol ganglion cells. Ganglion cells in the retinae of marmoset and macaque monkeys were injected with Neurobiotin in a live in vitro retinal whole-mount preparation. Retinal pieces were then processed with an antibody against the alpha 1 subunit of the glycine receptor. Strong punctate immunoreactivity indicative of synaptic localization is present in the ON and OFF sublamina of the inner plexiform layer. Many of the immunoreactive puncta coincide with the dendrites of both midget and parasol ganglion cells. Immunoreactive puncta are present on distal and proximal dendrites of ON and OFF cells. These results suggest that ON and OFF midget and parasol cells do not differ with respect to the distribution of the alpha 1 subunit of the glycine receptor.