Detection of Oseltamivir Resistance during Treatment of 2009 H1N1 Influenza Virus Infection in Immunocompromised Patients: Utility of Cycle Threshold Values of Qualitative Real-Time Reverse Transcriptase PCR
Detection of Oseltamivir Resistance during Treatment of 2009 H1N1 Influenza Virus Infection in Immunocompromised Patients: Utility of Cycle Threshold Values of Qualitative Real-Time Reverse Transcriptase PCR
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DOI：
10.1128/jcm.01190-10
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发表时间：
2010-11-01
影响因子：
9.4
通讯作者：
Alangaden, George J.
中科院分区：
文献类型：
--
作者：
Mehta, Tejal;McGrath, Eric;Alangaden, George J.
两名免疫功能低下的2009年H1N1流感肺炎患者尽管接受了奥司他韦治疗，但病毒脱落超过5周。在呼吸道标本的系列定性实时逆转录酶PCR（rRT-PCR）中观察到的循环阈值下降或持续较低，这意味着病毒载量增加和可能的耐药性。后来通过焦磷酸测序证实了奥司他韦耐药性。
Two immunocompromised patients with 2009 H1N1 influenza pneumonia had viral shedding for over 5 weeks despite therapy with oseltamivir. Declining or persistently low cycle threshold values noted on serial qualitative real-time reverse transcriptase PCR (rRT-PCR) of respiratory specimens implied increasing viral load and probable drug resistance. Oseltamivir resistance was later confirmed by pyrosequencing.