Pattern of cardiorespiratory afferent convergence to solitary tract neurons driven by pulmonary vagal C-fiber stimulation in the mouse
Pattern of cardiorespiratory afferent convergence to solitary tract neurons driven by pulmonary vagal C-fiber stimulation in the mouse
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DOI：
10.1152/jn.1998.79.5.2365
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发表时间：
1998-05-01
影响因子：
2.5
通讯作者：
Paton, JFR
中科院分区：
文献类型：
--
作者：
Paton, JFR
在成年小鼠的动脉灌注工作心脑干制剂中，研究了来自肺迷走神经 C 纤维（或 J 型受体）的信号与来自心脏、外周化学感受器和压力感受器传入孤束核 (NTS) 内神经元的信号的中心整合。右心房注射苯双胍（PBG）可激发肺迷走神经C纤维，而左心室注射藜芦定（1-3μg/kg）或使用留置套管机械地扩张左心室（20-50μg/kg）可刺激心脏受体。主动脉注射氰化钠可激活颈动脉体化学感受器，而增加动脉灌注压可刺激压力感受器。肺 C 纤维和心脏、化疗和压力感受器的刺激都会产生反射性心动过缓 (23-133 bpm)。从膈神经记录的中枢呼吸活动通过刺激肺 C 纤维以及心脏和压力感受器而被抑制，但在化学感受器刺激期间其幅度和频率增强。肺部 C 纤维刺激显示出峰值放电频率高达 42 Hz（15 +/- 2.2 Hz，平均值 +/- SE）的递减放电，持续 8.8 +/- 0.9 秒，27 个 NTS 神经元被兴奋，3 个被抑制。这些反应发生了
The central integration of signals from pulmonary vagal C-fibers (or type-J receptors) with those arising from cardiac, peripheral chemoreceptor, and baroreceptor afferents to neurons within the nucleus of the solitary tract (NTS) was studied in an arterially perfused working heart-brain stem preparation of adult mouse. Pulmonary vagal C-fibers were excited by right atrial injection of phenylbiguanide (PBG) while cardiac receptors were stimulated by left ventricular injection of veratridine (1-3 mu g/kg) or mechanically by distension of the left ventricle (20-50 mu 1 perfusate) using an indwelling cannula. Carotid body chemoreceptors were activated by aortic injection of Na cyanide, whereas baroreceptors were stimulated by increasing arterial perfusion pressure. Stimulation of pulmonary C-fibers and cardiac, chemo-, and baroreceptors all produced a reflex bradycardia (23-133 bpm). Central respiratory activity, as recorded from the phrenic nerve, was depressed by stimulating pulmonary C-fibers and cardiac and baroreceptors but enhanced in amplitude and frequency during chemoreceptor stimulation. Twenty-seven NTS neurons were excited and three were inhibited after pulmonary C-fiber stimulation displaying decrementing discharges with a peak firing frequency of up to 42 Hz (15 +/- 2.2 Hz, mean +/- SE) that lasted for 8.8 +/- 0.9 s. These responses occurred