Reduction of prion infectivity in packed red blood cells
Reduction of prion infectivity in packed red blood cells
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DOI：
10.1016/j.bbrc.2008.09.141
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发表时间：
2008-12-12
影响因子：
3.1
通讯作者：
Soto, Claudio
中科院分区：
文献类型：
--
作者：
Morales, Rodrigo;Buytaert-Hoefen, Kimberley A.;Soto, Claudio
一种新的克雅氏病变种(VCJD)与食用被普恩病毒污染的牛肉之间的联系，以及最近的发现表明vCJD可以通过输血传播，这引起了公众对卫生问题的关注。目前，还没有一种可靠的测试来识别血液样本中的普恩病毒。本研究的目的是评价利用细胞分离和洗涤设备通过简单的洗涤程序去除红细胞悬液中的瘙痒病病毒蛋白(PrPSc)和传染性的可能性。通过Western印迹、PMCA和感染性生物测定来评估病毒对病毒的去除程度。我们的结果显示，所有使用的技术都能实质性地去除传染性病毒(>；=3对数传染性)。这些数据表明，红细胞制剂中存在的大量传染性可以通过简单的洗涤程序去除。这项技术可能会提高血液产品的安全性，并降低Pron疾病进一步传播的风险。(C)2008 Elsevier Inc.保留所有权利。
The link between a new variant form of Creutzfeldt-Jakob disease (vCJD) and the consumption of prion contaminated cattle meat as well as recent findings showing that vCJD can be transmitted by blood transfusion have raised public health concerns. Currently, a reliable test to identify prions in blood samples is not available. The purpose of this study was to evaluate the possibility to remove scrapie prion protein (PrPSc) and infectivity from red blood cell (RBC) suspensions by a simple washing procedure using a cell separation and washing device. The extent of prion removal was assessed by Western blot, PMCA and infectivity bioassays. Our results revealed a substantial removal of infectious prions (>= 3 logs of infectivity) by all techniques used. These data suggest that a significant amount of infectivity present in RBC preparations can be removed by a simple washing procedure. This technology may lead to increased safety of blood products and reduce the risk of further propagation of prion diseases. (C) 2008 Elsevier Inc. All rights reserved.