A reproducible, rapid and inexpensive Folin-Ciocalteu micro-method in determining phenolics of plant methanol extracts
A reproducible, rapid and inexpensive Folin-Ciocalteu micro-method in determining phenolics of plant methanol extracts
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DOI：
10.1016/j.microc.2008.08.011
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发表时间：
2009-01-01
影响因子：
4.8
通讯作者：
Lattanzio, Vincenzo
中科院分区：
文献类型：
--
作者：
Cicco, Nunzia;Lanorte, Maria T.;Lattanzio, Vincenzo
本文提出了一种新的时间、温度、碱和乙醇的组合，用于分光光度法测定植物甲醇提取物中微量酚类化合物。它是经典Folin-Ciocalteu（F-C）方法的变体，但对反应条件进行了优化，以消除测定中的甲醇干扰。使用5%（w/v）碳酸钠溶液，在40 ℃下，将醇浓度和反应时间限值评价为4%甲醇（v/v）和20 min。这种F-C微量方法是可重复的，快速的，便宜的，特别有用的，如果它与大量的样品或在小规模上工作，如在建立一个实验过程中的酒精提取。(C)2008 Elsevier B. V.保留所有权利。
A new combination among time, temperature, alkali and alcohol is described for the spectrophotometric determination of small concentrations of phenolics in methanol extracts from plant. It is a variation of the classical Folin-Ciocalteu (F-C) method, but the reaction conditions are optimized in order to eliminate methanol interferences in the assay. Alcohol concentration and reaction time limits have been evaluated as 4% methanol (v/v) and 20 min at 40 degrees C, using a 5% (w/v) sodium carbonate solution. This F-C micro-method is reproducible, quick, inexpensive and particularly helpful if it works with numerous samples or on a small scale, such as during the setting up of an experimental procedure of alcoholic extractions. (C) 2008 Elsevier B.V. All rights reserved.