Expression of calcium-binding S100 proteins A4 and A6 in regions of the epithelial Sac associated with the onset of hair follicle regeneration
Expression of calcium-binding S100 proteins A4 and A6 in regions of the epithelial Sac associated with the onset of hair follicle regeneration
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DOI：
10.1046/j.0022-202x.2001.01369.x
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发表时间：
2001-06-01
影响因子：
6.5
通讯作者：
Kizawa, K
中科院分区：
文献类型：
--
作者：
Ito, M;Kizawa, K
为了阐明毛发再生的启动机制，我们表征了转移和细胞生长相关的钙结合S100蛋白在再生毛囊上皮中的表达。在小鼠毛囊的上皮囊区（隆突区和毛胚）发现了S100 A4和S100 A6的毛发周期依赖性表达。S100 A4蛋白定位仅限于隆突区，假定的滤泡干细胞居住的区域，在退行期-休止期-生长期过渡期，而S100 A6蛋白分布在整个上皮囊区在生长期阶段。然而，在进入生长期之前，两种S100 mRNA在上皮囊中均上调。尽管拔毛后在隆突区域诱导了广泛的细胞死亡，但在毛胚中转录S100 A6后启动了一个新的毛发周期。在创伤刺激后，外根鞘和表皮基底层表达S100 A6 mRNA之前过度增殖。一旦上皮囊被诱导转录两个S100基因，静息卵泡随之复壮。这些结果表明，S100 A4和S100 A6可能在卵泡再生开始时干细胞的活化中起重要作用。
In order to elucidate the onset mechanisms of hair regrowth, we characterized the expression of metastasis and cell-growth-associated calcium-binding S100 proteins in the regenerating follicular epithelium, Hair-cycle-dependent expression of S100A4 and S100A6 was found in the epithelial sac regions (bulge area and hair germ) of mouse pelage follicles. Protein localization of S100A4 was confined to the bulge area, the region where the presumed follicular stem cells reside, during the catagen-telogen-anagen transitional periods, whereas S100A6 protein was distributed throughout the epithelial sac regions during anagen phase. Prior to entering anagen phase, however, both S100 mRNAs were upregulated in the epithelial sac. Despite the induction of extensive cell death in the bulge region after plucking, a new hair cycle was initiated following transcription of S100A6 in the hair germ. Upon wounding stimuli, both the outer root sheath and the basal layer of epidermis expressed S100A6 rnRNA prior to hyper-proliferation. Once the epithelial sac was induced to transcribe both S100 genes, resting follicles were concomitantly rejuvenated. These results suggest that S100A4 and S100A6 might play important roles in the activation of stem cells at the onset of follicle regeneration.