Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing.
Lignocellulose-responsive bacteria in a southern California salt marsh identified by stable isotope probing.
复制标题
DOI：
10.3389/fmicb.2014.00263
复制
发表时间：
2014
影响因子：
5.2
通讯作者：
Dillon JG
中科院分区：
文献类型：
--
作者：
Darjany LE;Whitcraft CR;Dillon JG
微生物的碳循环已被认为是沿海湿地有机物分解和输出的主要机制，但对特定分解者群体的功能多样性（例如，细菌）在盐沼底栖营养结构中的作用。事实上，盐沼沉积物细菌在盐沼底栖食物网途径中的多样性和功能作用在很大程度上仍然处于黑盒子中。我们使用DNA稳定同位素探针（SIP）利用13 C-标记的木质纤维素作为代理，以评估的命运的大型植物来源的碳在底栖盐沼细菌群落。总体而言，146个细菌物种被检测到使用SIP，其中只有12个谱系之间的富集和非富集社区共享。来自13 C标记的群落的丰富类群包括脱硫八叠球菌属、螺旋体属和康氏菌属。这项研究是第一个使用重标记的木质纤维素，以确定细菌负责大型植物的碳利用盐沼沉积物，并将允许未来的研究，以针对特定的谱系，以阐明其在盐沼碳循环的作用，并最终帮助我们了解盐沼的潜力，以存储碳。
Carbon cycling by microbes has been recognized as the main mechanism of organic matter decomposition and export in coastal wetlands, yet very little is known about the functional diversity of specific groups of decomposers (e.g., bacteria) in salt marsh benthic trophic structure. Indeed, salt marsh sediment bacteria remain largely in a black box in terms of their diversity and functional roles within salt marsh benthic food web pathways. We used DNA stable isotope probing (SIP) utilizing 13C-labeled lignocellulose as a proxy to evaluate the fate of macrophyte-derived carbon in benthic salt marsh bacterial communities. Overall, 146 bacterial species were detected using SIP, of which only 12 lineages were shared between enriched and non-enriched communities. Abundant groups from the 13C-labeled community included Desulfosarcina, Spirochaeta, and Kangiella. This study is the first to use heavy-labeled lignocellulose to identify bacteria responsible for macrophyte carbon utilization in salt marsh sediments and will allow future studies to target specific lineages to elucidate their role in salt marsh carbon cycling and ultimately aid our understanding of the potential of salt marshes to store carbon.
登录
查看更多内容
影响因子：
2.7
作者：
Bushaw-Newton, Karen L.;Kreeger, Danielle A.;Velinsky, David J.
通讯作者：
Velinsky, David J.
影响因子：
2.1
作者：
BOUYOUCOS, GJ
通讯作者：
BOUYOUCOS, GJ
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
影响因子：
5.1
作者：
Haichar, Feth el Zahar;Achouak, Wafa;Berge, Odile
通讯作者：
Berge, Odile
影响因子：
3.6
作者：
DeAngelis, Kristen M.;Gladden, John M.;Hazen, Terry C.
通讯作者：
Hazen, Terry C.