Fine-mapping and candidate gene analysis of qFL-c10-1 controlling fiber length in upland cotton (Gossypium hirsutum L.).
Fine-mapping and candidate gene analysis of qFL-c10-1 controlling fiber length in upland cotton (Gossypium hirsutum L.).
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DOI：
10.1007/s00122-022-04233-6
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发表时间：
2022-10
期刊：
影响因子：
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通讯作者：
Rui Zhang;Chao Shen;De Zhu;Yu Le;Nian Wang;Yuanxue Li;Xianlong Zhang;Zhongxu Lin
中科院分区：
文献类型：
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作者：
Rui Zhang;Chao Shen;De Zhu;Yu Le;Nian Wang;Yuanxue Li;Xianlong Zhang;Zhongxu Lin
关键信息一个纤维长度QTL，qFL-c10-1，被精细定位到一个96.5 kb的区域，包含一个基因，还没有在plants.AbstractFiber长度是棉花纤维品质的重要组成部分，这是与其他质量特性，如纤维强度，纤维成熟度和细度。在前期的研究中，我们在高地棉重组自交系（RIL）群体中，利用冀棉5号与DH 962杂交，在A10染色体上发现了一个稳定的控制纤维长度的QTLqFL-c10- 1。为了对qFL-c10 -1基因进行精细定位，以重组系DJ 61与冀棉5号杂交的F2代群体为材料，构建了1081株的F2代群体。通过连锁分析和后代重组实验，将qFL-c10- 1定位在一个96.5kb的基因组区域内，该区域仅包含一个植物中未描述的基因Ghir_A10G022020（命名为GhFL 10）。与冀棉5号相比，DJ 61的启动子区有一个214 bp的缺失。实时荧光定量PCR（qRT-PCR）和亲本序列比较分析表明，GhFL 10是qFL-c10 -1最有希望的候选基因。根据RNA-seq、酵母双杂交和双分子荧光互补技术（BiFC），我们推测GhFL 10与NF-YA转录因子相互作用，负调控纤维的伸长。
Key messageA fiber length QTL,qFL-c10-1, was fine-mapped to a 96.5-kb region containing one gene that has not been characterized in plants.AbstractFiber length is an important component of cotton fiber quality, which is associated with other quality properties such as fiber strength, fiber maturity, and fineness. In our previous studies, a stable QTLqFL-c10-1controlling fiber length had been identified on chromosome A10 in an upland cotton recombinant inbred line (RIL) population from a cross between Jimian5 and DH962. To fine-mapqFL-c10-1, an F2population with 1081 individual plants from a cross between a recombinant line DJ61 and Jimian5 was established. Using linkage analysis and progeny recombination experiment,qFL-c10-1was mapped into a 96.5-kb genomic region that just contained one proper transcriptGhir_A10G022020(described asGhFL10), an undescribed gene in plants. One 214-bp deletion was identified in the promoter region of DJ61 compared with Jimian5. Quantitative real-time PCR (qRT-PCR) and comparative analysis of parental sequences suggested thatGhFL10was the most promising candidate gene forqFL-c10-1. According to RNA-seq, yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC), we speculate thatGhFL10interacts with NF-YA transcription factors to negatively regulate fiber elongation.