A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system
A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system
复制标题
一种基于唾液酸-苯硼酸识别系统的快速、生物相容性活病毒标记方法
DOI：
10.1007/s00216-014-7651-9
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发表时间：
2014-04-01
影响因子：
4.3
通讯作者：
Xie, Hai-Yan
中科院分区：
文献类型：
--
作者：
Huang, Li-Li;Jin, Yong-Jie;Xie, Hai-Yan
唾液酸（SA）-苯基硼酸（PBA）识别系统在生物共轭领域特别受关注，因为它简单、快速、高效且具有生物相容性。在本文中，我们报告了一种利用 SA-PBA 识别系统用量子点 (QD) 可逆标记活病毒的新方法。量子点最初用 PBA (QDs-PBA) 进行修饰，以将其靶向水疱性口炎病毒 (VSV) 的表面，该病毒的包膜上含有丰富的 SA。 QDs-PBA 具有良好的单分散性和强荧光，只需在 37°C 下与天然 VSV 孵育 10 分钟即可与 VSV 结合，产生能够在单个病毒体水平上成像的 QDs-VSV。标记效率达到83±4.3%（平均值±SD）；同时，标记病毒的活性和识别能力受到的影响最小。该方法简单、快速、可逆。这项工作将病毒标记的发展推向了一个新的台阶。也就是说，天然病毒无需任何修饰即可可逆标记。图最初，3-氨基苯基硼酸（APBA）与QDs-COOH反应制备PBA封端的QDs（QDs-PBA）。然后，基于唾液酸-苯硼酸识别系统，可以用QDs-PBA一步高效、特异地标记包膜表面富含唾液酸（SA）的水泡性口炎病毒（VSV）。标记效率达到(83±4.3%)(平均值±SD)。标记的 VSA (QDs-VSV) 仍然具有传染性，并且能够在单个病毒颗粒水平上成像
The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification.FigureInitially, the 3-amino-phenylboronic acid (APBA) reacted with QDs-COOH to prepare the PBA capped QDs (QDs-PBA). Then, the vesicular stomatitis virus (VSV), on which the surface of the envelope is abundant with sialic acid (SA), could be labeled efficiently and specifically with QDs-PBA in one step based on sialic acid-phenylboronic acid recognition system. The labeling efficiency attained to (83 ± 4.3 %) (mean ± SD). The labeled VSA (QDs-VSV) was still infectious and capable of being imaged at the single virion level