Aptamer cell sensor based on porous graphene oxide decorated ion-selective-electrode: Double sensing platform for cell and ion
Aptamer cell sensor based on porous graphene oxide decorated ion-selective-electrode: Double sensing platform for cell and ion
复制标题
基于多孔氧化石墨烯修饰离子选择电极的适配体细胞传感器：细胞和离子双传感平台
DOI：
10.1016/j.bios.2018.06.021
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发表时间：
2018
影响因子：
12.6
通讯作者：
Jia Yunfang
中科院分区：
文献类型：
--
作者：
Zhang Rong;Gu Yajun;Wang Zhongrong;Li Yueguo;Fan Qingjie;Jia Yunfang
受基于离子选择性电极（伊势）的细胞离子检测技术的启发，提出了一种基于多孔氧化石墨烯（PGO）修饰的伊势（PGO-ISE）的适配体捕获和伊势转导（AC&IT）策略，并以AS 1411靶向A549细胞检测和碘离子选择性电极作为概念验证，考察了其在细胞和离子检测中的性能。首先，采用改进的Hummers法从石墨片上剥离GO，然后通过硫脲水热交联得到三维网状PGO，通过扫描电镜、紫外可见吸收光谱和X射线光电子能谱对其进行表征，并通过显微镜评价其对细胞捕获的增强作用。然后，通过在伊势表面滴涂PGO膜并随后共价锚定AS 1411来构建PGO-ISE。利用自行研制的ISE测试系统对不同状态的伊势（空白、PGO涂层、AS 1411锚定和A549捕获）进行了电化学测试。结果表明，在缓冲液中的最佳细胞灵敏度为-25.21 mV/log 10 CA 549（R2= 0.91），在血液中的分辨率为10个细胞/ml。有趣的是，由于PGO的支架对离子载体的保护，I-敏感性保持为− 42.98 mV/pI（R2= 0.95，pI = -log 10（CI））。从伊势的基本原理出发，对双敏感现象进行了理论解释。基于PGO-ISE的核酸适体细胞传感器有望成为生物医学研究的一种新的实验手段。
Enlightened by the emerging cell-ion detection based on ion-selective-electrode (ISE), an aptamer capturing and ISE transducing (AC&IT) strategy is proposed on the porous graphene oxide (PGO) decorated ISE (PGO-ISE), its performances in both cell and ion detections are examined by use of AS1411 targeted A549 cell detection and iodide-ISE as proof-of-concept. Firstly, GO flakes, exfoliated from graphite by modified Hummers method, are cross-linked by thiourea mediated hydrothermal process, to 3-dimension networked PGO which is identified by scanning-electron-microscope, UV–visible absorbance and X-ray photoelectron spectroscopy; its enhancing effect for cell capturing is evaluated by microscopy. Then, PGO-ISE is constructed by drop-coating PGO film on the surface of ISE and followed by covalently anchoring AS1411. Electrochemistry measurements for different state ISE (blank, PGO coated, AS1411 anchored and A549 captured) are performed by our home-made ISE-measuring system. It is demonstrated that the best cell-sensitivity in buffer is − 25.21 mV/log10CA549(R2= 0.91), resolution in blood is 10 cells/ml. Interestingly, due to PGO's scaffold protection to the ionophore, I--sensitivity is preserved as − 42.98 mV/pI (R2= 0.95, pI = -log10(CI)). Theoretical explanations are provided for the double-sensing phenomenon according to basic ISE principle. It is believed the PGO-ISE based aptamer cell sensor will be a promising experimental means for biomedical researches.