MYOCARDIAL RECOVERY DURING POSTISCHEMIC REPERFUSION - EFFECTS OF NIFEDIPINE, CALCIUM AND MAGNESIUM
MYOCARDIAL RECOVERY DURING POSTISCHEMIC REPERFUSION - EFFECTS OF NIFEDIPINE, CALCIUM AND MAGNESIUM
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DOI：
10.1016/s0022-2828(86)80914-2
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发表时间：
1986-05-01
影响因子：
5
通讯作者：
VISIOLI, O
中科院分区：
文献类型：
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作者：
FERRARI, R;ALBERTINI, A;VISIOLI, O
我们研究了缺血后再灌注时引入的各种干预措施对离体灌注兔心脏的机械活动、组织和线粒体钙、线粒体功能和组织ATP和CP的影响。这些干预措施是硝苯地平，低钙（0.75 mM，0.15 mM和0.05 mM），高镁（15 mM）和高镁（15 mM）与低钙（0.75 mM）。通过阻断冠状动脉血流60分钟，然后再灌注30分钟来诱导缺血。结果表明，硝苯地平在再灌注期间给药时没有保护作用，而在缺血前给药时是有益的。根据钙减少的程度，在再灌注期间降低灌注液的钙含量可能是有利的。高镁再灌注降低线粒体钙和维持线粒体ATP产生能力，但未能修改组织钙和舒张压的增加。在高镁存在下降低钙含量导致更好的保护。提示再灌注条件可能影响心肌的恢复能力。
We studied the effects of various interventions introduced at the time of post-ischaemic reperfusion on mechanical activity, tissue and mitochondrial calcium, mitochondrial function and tissue ATP and CP of isolated perfused rabbit hearts. These interventions were nifedipine, low calcium (0.75 mM, 0.15 mM and 0.05 mM), high magnesium (15 mM) and high magnesium (15 mM) with low calcium (0.75 mM). Ischemia was induced by abolishing coronary flow for 60 min, followed by 30 min of reperfusion. The results indicate that nifedipine when given during reperfusion had no protective effect, whilst it was beneficial when administered before ischaemia. Lowering calcium content of the perfusate during reperfusion may be advantageous, depending on the degree of calcium reduction. Reperfusion with high magnesium reduced the mitochondrial calcium and maintained the mitochondrial ATP-producing capacity but failed to modify the increase of tissue calcium and of diastolic pressure. Lowering calcium content in the presence of high magnesium resulted in better protection. These data suggest that the conditions of reperfusion may influence the capacity of myocardial recovery.