Trapping proteins on nanopores by dielectrophoresis
Trapping proteins on nanopores by dielectrophoresis
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DOI：
10.1063/5.0144564
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发表时间：
2023-04-28
影响因子：
3.2
通讯作者：
Matyushov, Dmitry V.
中科院分区：
文献类型：
--
作者：
Colburn, Taylor;Matyushov, Dmitry V.
近年来，由于介电电泳在水溶液中对溶质（如蛋白质）的分选、操纵和捕获的潜力，对介电电泳现象的兴趣已经获得了显著的关注。几十年来，蛋白质介电电泳被认为是不可能的，因为预测的由实验可获得的场强产生的力的大小不能超过热能。这一结论是从应用于介电泳力的主要Clausius-Mossotti（CM）敏感性得出的。然而，介电界面极化导致的CM结果不占一个大的蛋白质偶极矩，这是负责的偶极机制的介电电泳竞争的CM诱导机制的情况下，蛋白质的三至四个数量级。在这里，我们提出了一个明确的几何形状内的偶极磁化率可以进行测试。电场和介电电泳力的明确计算，并探讨的捕获距离的依赖性上的施加的电场的强度。一些可观察到的区别之间的偶极和诱导机制被确定。
Interest in the phenomenon of dielectrophoresis has gained significant attention in recent years due to its potential for sorting, manipulation, and trapping of solutes, such as proteins, in aqueous solutions. For many decades, protein dielectrophoresis was considered impossible, as the predicted magnitude of the force arising from experimentally accessible field strengths could not out-compete thermal energy. This conclusion was drawn from the mainstay Clausius-Mossotti (CM) susceptibility applied to the dielectrophoretic force. However, dielectric interfacial polarization leading to the CM result does not account for a large protein dipole moment that is responsible for the dipolar mechanism of dielectrophoresis outcompeting the CM induction mechanism by three to four orders of magnitude in the case of proteins. Here, we propose an explicit geometry within which the dipolar susceptibility may be put to the test. The electric field and dielectrophoretic force are explicitly calculated, and the dependence of the trapping distance on the strength of the applied field is explored. A number of observable distinctions between the dipolar and induction mechanisms are identified.