Induction and morphogenesis of chlamydospores in an agerminative variant of Candida albicans.
Induction and morphogenesis of chlamydospores in an agerminative variant of Candida albicans.
复制标题
白色念珠菌繁殖变体中厚垣孢子的诱导和形态发生。
DOI：
10.1080/00362178385380081
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Antonio Cassone
中科院分区：
文献类型：
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作者：
A. Torosantucci;Antonio Cassone
不形成芽管的白色念珠菌菌株被赋予在适当的环境条件下大量生产厚垣孢子的显著能力。厚垣孢子的发育由N-乙酰基己糖胺，特别是N-乙酰基-D-葡萄糖胺诱导，这种诱导不依赖于非特异性利用N-乙酰基己糖胺作为代谢源，也不与芽管形成的诱导相关。厚垣孢子在N-乙酰己糖胺-琼脂培养基中的形成通过增殖阶段（10-12 h）发生，该增殖阶段由导致短的“假菌丝样”结构的几个出芽循环组成，随后大多数细胞逐渐分化成年轻的厚垣孢子（16-18 h），该年轻的厚垣孢子在36-48 h内完全成熟。不同菌株间厚垣孢子的形成存在显著差异。但当诱导时，孢子形成的形态和动力学在所有菌株中是相同的。这项研究表明，厚垣孢子的形成不一定与菌丝体阶段，并表明，N-乙酰氨基己糖可能会诱导孢子形成通过控制内源性代谢，而不是通过自己的代谢产物。
A strain of Candida albicans that did not form germ tubes was endowed with a pronounced ability for massive production of chlamydospores under appropriate environmental conditions. Development of chlamydospores was induced by N-acetyl hexosamines, especially N-acetyl-D-glucosamine, and this induction did not depend on non-specific utilization of N-acetyl hexosamines as metabolic sources nor did it correlate with induction of germ-tube formation. Formation of chlamydospores in N-acetyl hexosamine-agar medium occurs through a multiplication stage (10-12 h) consisting of a few cycles of budding leading to short, "pseudo-hypha-like" structures, followed by progressive differentiation of most cells into young chlamydospores (16-18 h) which go to complete maturation in 36-48 h. There were marked differences in chlamydospore formation among different strains of C. albicans but, when induced, the morphology and kinetics of sporulation were identical in all strains. This study shows that chlamydospore formation is not necessarily associated with the mycelial phase and suggests that N-acetyl hexosamines may induce sporulation by controlling endogenous metabolism rather than through products of their own metabolism.