Characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolated from municipal and hospital wastewaters in Japan
Characterization of extended-spectrum beta-lactamase-producing Escherichia coli isolated from municipal and hospital wastewaters in Japan
复制标题
从日本市政和医院废水中分离出的产广谱 β-内酰胺酶大肠杆菌的特征
DOI：
10.1016/j.jgar.2023.02.002
复制
发表时间：
2023
影响因子：
4.6
通讯作者：
Toru Watanabe
中科院分区：
文献类型：
--
作者：
Rio Shibuki;Masateru Nishiyama;Masaya Mori;Hiroaki Baba;Hajime Kanamori;Toru Watanabe
目的通过检测产超广谱β-内酰胺酶（ESBL）大肠埃希菌（ESBL-EC）的耐药基因（ARG）和药敏试验，了解其在城市污水和医院污水中的分布特征。为了确定来源的ESBL-EC，多位点序列分型和分型质粒，可能携带产ESBL-producing genes were conducted.Methodswastewater was sampling twice a month from February 2019 to February 2020 from a municipal wastewater treatment plant and hospital located in a city in Northeast Japan.在整个研究期间，279和37株ESBL-EC从城市和医院废水分离，results.ResultsAll 316株耐氨苄西林和头孢噻肟和敏感亚胺培南和替加环素。几乎所有（98.1%）ESBL-EC分离株均具有blaCTX-M，其中blaCTX-M-9组检测频率最高（62.3%）。多位点序列分型揭示了更高的序列类型（ST）的多样性，在城市污水中的菌株比那些从医院废水，虽然ST 131，最近引起医院和社区相关的感染，在这两种类型的废水占主导地位。所有的ST 131菌株拥有的IncFII质粒，这是经常报告携带blaCTX-M.ConclusionsThese结果表明，健康人携带临床上重要的耐药细菌和ARGs，激励常规监测城市污水检测这种耐药细菌和ARGs从各种来源支持的ST在本研究中的高多样性。
ObjectivesThe purpose of this study was to characterize the strains of extended-spectrum beta-lactamase (ESBL)-producingEscherichia coli(ESBL-EC) isolated from municipal and hospital wastewater by detecting antibiotic resistance genes (ARGs) as well as antibiotic susceptibility. To identify the source of ESBL-EC, multi-locus sequence typing and typing plasmids that may carry ESBL-producing genes were conducted.MethodsWastewater was sampled twice a month from February 2019 to February 2020 from a municipal wastewater treatment plant and hospital located in a city in northeastern Japan. Throughout the study period, 279 and 37 strains of ESBL-EC were isolated from municipal and hospital wastewater, respectively.ResultsAll 316 isolates were resistant to ampicillin and cefotaxime and susceptible to imipenem and tigecycline. Almost all (98.1%) of the ESBL-EC isolates possessedblaCTX-M, and theblaCTX-M-9group was detected most frequently (62.3%). Multi-locus sequence typing revealed a higher diversity of sequence types (STs) in the isolates from municipal wastewater than in those from hospital wastewater; although ST131, which recently caused nosocomial- and community-associated infections worldwide, was dominant in both types of wastewater. All ST131 isolates possessed the IncFII plasmid, which is often reported to carryblaCTX-M.ConclusionsThese results demonstrated that healthy people carry clinically important antibiotic-resistant bacteria and ARGs, motivating routine monitoring of municipal wastewater to detect such antibiotic-resistant bacteria and ARGs from a variety of sources supported by the high diversity of STs in the present study.