Cloning of a Streptococcus sobrinus oligo-isomaltosaccharide synthase gene and characterization of its product.
Cloning of a Streptococcus sobrinus oligo-isomaltosaccharide synthase gene and characterization of its product.
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远缘链球菌低聚异麦芽糖合酶基因的克隆及其产物的表征。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
Hisashi Takiguchi
中科院分区：
文献类型：
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作者：
M. Hayakawa;K. Fukushima;Yoshimitsu Abiko;T. Ikeda;Hisashi Takiguchi
使用噬菌体λ L47.1和质粒载体pACYC 184将编码葡糖基转移酶（GTF）-S的远缘链球菌基因克隆到大肠杆菌中。获得的MD 124克隆表达155 kDa GTF-S，其不与任何抗GTF-S1、-S2和-I酶的抗血清反应。从MD 124细胞提取物中均匀纯化重组酶（命名为rGTF-S3）并进行表征。纯化的rGTF-S3从蔗糖合成引物独立的α-1，6-连接的线性寡糖。对蔗糖浓度的依赖性呈双相性，Km值分别为1.3和25 mM，除Km值外，其他性质与S.长叶藓
A Streptococcus sobrinus gene coding for a glucosyltransferase (GTF)-S was cloned into Escherichia coli, using the bacteriophage lambda L47.1 and the plasmid vector pACYC184. The MD124 clone obtained expressed a 155 kDa GTF-S which did not react with any antisera against GTF-S1, -S2 and -I enzymes. The recombinant enzyme (designated rGTF-S3) was homogeneously purified from the MD124 cell-extract and characterized. The purified rGTF-S3 synthesized primer-independently alpha-1,6-linked linear oligosaccharides from sucrose. The dependence upon the sucrose concentration was diphasic, and the respective Km values were 1.3 and 25 mM. The properties except the Km values were similar to those of oligo-isomaltosaccharide synthase from S. sobrinus AHT.