GEISHA: an in situ hybridization gene expression resource for the chicken embryo
GEISHA: an in situ hybridization gene expression resource for the chicken embryo
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DOI：
10.1159/000103162
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Antin, P. B.
中科院分区：
文献类型：
--
作者：
Darnell, D. K.;Kaur, S.;Antin, P. B.
生物医学和农业鸟类研究的一个重要和持续的焦点是了解基因功能，其中很大一部分基因仍然未知。第一步是确定基因在发育过程中和成人中何时何地表达。整体原位杂交提供了精确的空间和时间分辨率的基因表达在整个胚胎，和一个全面的分析和集中的原位杂交信息库将提供一个有价值的研究工具。GEISHA项目（gallus expression in situ hybridization analysis，鸡表达原位杂交分析）的启动是为了探索高通量原位杂交技术在鸡胚胎基因发现和注释中的应用，并为原位杂交信息提供统一的信息库。本报告介绍了一个新的GEISHA数据库和用户界面（www.geisha.arizona.edu）的设计和实施，并说明了其实用程序的研究人员在生物医学和家禽科学界。还介绍了鸡胚胎中microRNA表达的高通量筛选获得的结果。版权所有（c）2007 S. Karger AG，巴塞尔
An important and ongoing focus of biomedical and agricultural avian research is to understand gene function, which for a significant fraction of genes remains unknown. A first step is to determine when and where genes are expressed during development and in the adult. Whole mount in situ hybridization gives precise spatial and temporal resolution of gene expression throughout an embryo, and a comprehensive analysis and centralized repository of in situ hybridization information would provide a valuable research tool. The GEISHA project (gallus expression in situ hybridization analysis) was initiated to explore the utility of using high-throughput in situ hybridization as a means for gene discovery and annotation in chicken embryos, and to provide a unified repository for in situ hybridization information. This report describes the design and implementation of a new GEISHA database and user interface (www.geisha.arizona.edu), and illustrates its utility for researchers in the biomedical and poultry science communities. Results obtained from a high throughput screen of microRNA expression in chicken embryos are also presented. Copyright (c) 2007 S. Karger AG, Basel