Ciliary function and motor protein composition of human fallopian tubes
Ciliary function and motor protein composition of human fallopian tubes
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DOI：
10.1093/humrep/dev227
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发表时间：
2015-12-01
影响因子：
6.1
通讯作者：
Omran, Heymut
中科院分区：
文献类型：
--
作者：
Raidt, Johanna;Werner, Claudius;Omran, Heymut
研究问题：人输卵管纤毛的运动蛋白成分和功能是什么？总结回答：虽然运动蛋白的组成和功能的人类FT纤毛类似的呼吸纤毛，女性原发性纤毛运动障碍（PCD）不一定是infertial.What是已知的：FT内衬多个运动纤毛，这表明了9 + 2超微结构的透射电子显微镜。病例报告表明PCD患者的生育力低下和异位妊娠的发生率增加，PCD是一种以运动纤毛和鞭毛功能障碍为特征的疾病。本研究包括对2012年4月至2013年12月招募的5名健康女性的人FT标本进行的观察性实验室研究和对临床PCD数据库的描述性观察性回顾性分析。材料，地点，方法：人FT组织取自5名健康女性在剖腹产时输卵管结扎后。使用针对动力蛋白臂和连接蛋白-动力蛋白调节复合物亚基的抗体通过免疫荧光显微镜评估马达蛋白组成。通过高速视频显微镜分析纤毛运动。回顾性搜索我们的数据库的PCD个人进行了信息的概念和birthing.Main结果和角色的机会：运动蛋白组成的人FT纤毛是相同的呼吸纤毛。FT纤毛显示协调跳动，导致液体流向子宫腔。我们确定了9个PCD个人呼吸纤毛严重功能障碍谁生下孩子后自然受孕。这表明纤毛跳动不是卵子运输的关键动力。局限性，预防原因：受影响PCD女性的FT纤毛无法用于分析。因此，仍有待证明的是，FT纤毛确实表现出与PCD个体中的呼吸纤毛相同的缺陷。需要全面的流行病学研究，以确定女性（分）生育力在PCD.WIDER影响的调查结果：知识的确切蛋白质组成和功能的FT纤毛将有助于更好地了解纤毛产生的流体流动在女性生殖的程度。这些发现对于后续PCD患者FT纤毛功能和蛋白质组成的研究具有重要意义。
STUDY QUESTION: What is the motor protein composition and function of human fallopian tube (FT) cilia?SUMMARY ANSWER: Although the motor protein composition and function of human FT cilia resemble that of respiratory cilia, females with primary ciliary dyskinesia (PCD) are not necessarily infertile.WHAT IS KNOWN ALREADY: FTs are lined with multiple motile cilia, which show a 9 + 2 ultrastructure by transmission electron microscopy. Case reports suggest an increased incidence of subfertility and ectopic pregnancy in women with PCD, a disease characterized by dysfunction of motile cilia and flagella.STUDY DESIGN, SIZE, DURATION: This study consisted of an observational laboratory study on human FT specimens from five healthy females recruited from April 2012 to December 2013 and a descriptive observational retrospective analysis of a clinical PCD database.PARTICIPANTS/MATERIALS, SETTING, METHODS: Human FT tissue was obtained from five healthy females after tubal ligation during caesarean delivery. Motor protein composition was assessed by immunofluorescence microscopy using antibodies against dynein arms and nexin-dynein regulatory complex subunits. Ciliary motility was analysed by high-speed video microscopy. A retrospective search of our database of PCD individuals was performed for information on conception and childbirth.MAIN RESULTS AND THE ROLE OF CHANCE: The motor protein composition of human FT cilia was identical to that of respiratory cilia. FT cilia showed coordinated beating, resulting in a directed fluid flow towards the uterine cavity. We identified nine PCD individuals with severe dysfunction of respiratory cilia who gave birth to children after spontaneous conception. This suggests that ciliary beating is not the key motor of ovum transport.LIMITATIONS, REASON FOR CAUTION: FT cilia of affected PCD females were not available for analysis. Thus, it remains to be proven that FT cilia indeed show the same defects as respiratory cilia in PCD individuals. Comprehensive epidemiological studies are needed to determine the extent of female (sub-) fertility in PCD.WIDER IMPLICATIONS OF THE FINDINGS: Knowledge of the exact protein composition and function of FT cilia will contribute to a better understanding of cilia-generated fluid flow in female reproduction. These findings are important for subsequent studies of function and protein composition of FT cilia in PCD patients.