An extracellular meiosis-promoting factor in Saccharomyces cerevisiae
An extracellular meiosis-promoting factor in Saccharomyces cerevisiae
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DOI：
10.1002/(sici)1097-0061(199805)14:7
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发表时间：
1998-05-01
期刊：
影响因子：
2.6
通讯作者：
Yamashita, I
中科院分区：
文献类型：
--
作者：
Hayashi, M;Ohkuni, K;Yamashita, I
酵母酿酒酵母的减数分裂和孢子形成一直被经典地视为响应于营养饥饿而发生的单细胞真核分化的实例。我们提出的证据表明，S。酿酒酵母产生一种细胞外因子，称为减数分裂促进因子（MEP），除了饥饿条件外，该因子是有效减数分裂和孢子形成所需的。该因子以细胞密度依赖性方式分泌和积累，使得在高密度细胞有效形成孢子的条件下，低密度细胞形成孢子较差。来自高密度产孢子细胞的条件培养基含有一种小阴离子分子，该分子具有细胞生长抑制活性，并在正常饥饿条件下刺激低密度细胞的孢子形成。这些结果表明，MEP介导的细胞之间的社会交流是减数分裂和孢子形成所必需的。(C)John Wiley & Sons，Ltd.
Meiosis and sporulation in the yeast Saccharomyces cerevisiae has been classically viewed as an example of unicellular, eukaryotic differentiation that occurs in response to nutritional starvation. We present evidence that S. cerevisiae produces an extracellular factor(s), called meiosis-promoting factor (MEP), that is required, in addition to starvation conditions, for efficient meiosis and sporulation. This factor is secreted and accumulates in a cell density-dependent fashion such that cells at a low density sporulate poorly under conditions in which cells at a high density sporulate efficiently. Conditioned medium from sporulating cells at a high density contains a small anionic molecule that has cytostatic activity and stimulates sporulation of cells at low density under a normal starvation condition. These results indicate that MEP-mediated social communication between cells is required for meiosis and sporulation. (C) 1998 John Wiley & Sons, Ltd.