Avian influenza virus and Newcastle disease virus (NDV) surveillance in commercial breeding farm in China and the characterization of Class I NDV isolates
Avian influenza virus and Newcastle disease virus (NDV) surveillance in commercial breeding farm in China and the characterization of Class I NDV isolates
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DOI：
10.1016/j.vetmic.2010.01.003
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发表时间：
2010-07-29
影响因子：
3.3
通讯作者：
Zhang, Xiumei
中科院分区：
文献类型：
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作者：
Hu, Beixia;Huang, Yanyan;Zhang, Xiumei
为了确定中国山东省鸭子中禽流感病毒（AIV）和新城疫病毒（NDV）的实际流行情况，2007年7月至2008年9月，对一个集约化养殖场的饲养鸭进行了广泛的监测研究。每月随机抽取30只健康的鸭子进行泄殖腔和气管拭子采集。所有拭子均呈甲型流感病毒阴性，而 2007 年 9 月收集的气管拭子和 100% 泄殖腔拭子中，新城疫病毒分离呈阳性，占 87.5%。从表面健康的鸭子的气管和泄殖腔拭子中回收了几种新城疫病毒分离株。根据 MDT 和 ICPI 测定，所有分离株均无致病性。对随机选取的4个分离株的HN基因和F基因可变区(nt 47-420)进行测序。 F基因的374 bp区域和HN基因的全长用于系统发育分析。根据 99.2-100% 的核苷酸序列同一性，四个分离株被鉴定为同一分离株，显示出与弱毒 I 类病毒更密切的系统发育关系。 F基因可变区(nt 47-420)与其他1类病毒的核苷酸差异为1.9-9.9%，与II类病毒的核苷酸差异为38.5-41.2%。这些分离株中F蛋白切割位点的氨基酸序列是112-ERQERL-117。这些菌株的HN基因全长1851bp，编码585个氨基酸。 HN基因核苷酸序列同源性分析表明，分离株与其他II类病毒之间存在2.0-4.2%的核苷酸差异，与II类病毒之间存在29.5-40.9%的差异。结果表明，这些分离株与疫苗株（LaSota）在系统发育上不相关。这项研究增加了对鸭中流感病毒和新城疫病毒生态学的了解，并强调在 AIV 和 NDV 流行持续且扩大的情况下持续监测的必要性。 (C) 2010 Elsevier B.V. 保留所有权利。
In order to determine the actual prevalence of avian influenza virus (AIV) and Newcastle disease virus (NDV) in ducks in Shandong province of China, extensive surveillance studies were carried out in the breeding ducks of an intensive farm from July 2007 to September 2008. Each month cloacal and tracheal swabs were taken from 30 randomly selected birds that appeared healthy. All of the swabs were negative for influenza A virus recovery, whereas 87.5% of tracheal swabs and 100% cloacal swabs collected in September 2007, were positive for Newcastle disease virus isolation. Several NDV isolates were recovered from tracheal and cloacal swabs of apparently healthy ducks. All of the isolates were apathogenic as determined by the MDT and ICPI. The HN gene and the variable region of F gene (nt 47-420) of four isolates selected at random were sequenced. A 374 bp region of F gene and the full length of HN gene were used for phylogenetic analysis. Four isolates were identified as the same isolate based on nucleotide sequences identities of 99.2-100%, displaying a closer phylogenetic relationship to lentogenic Class I viruses. There were 1.9-9.9% nucleotide differences between the isolates and other Class 1 virus in the variable region of F gene (nt 47-420), whereas there were 38.5-41.2% nucleotide difference between the isolates and Class II viruses. The amino acid sequences of the F protein cleavage sites in these isolates were 112-ERQERL-117. The full length of HN gene of these isolates was 1851 bp, coding 585 amino acids. The homology analysis of the nucleotide sequence of HN gene indicated that there were 2.0-4.2% nucleotide differences between the isolates and other Class II viruses, whereas there were 29.5-40.9% differences between the isolates and Class II viruses. The results shows that these isolates are not phylogenetically related to the vaccine strain (LaSota). This study adds to the understanding of the ecology of influenza viruses and Newcastle disease viruses in ducks and emphasizes the need for constant surveillance in times of an ongoing and expanding epidemic of AIV and NDV. (C) 2010 Elsevier B.V. All rights reserved.