Direct moxibustion exerts an analgesic effect on cervical spondylotic radiculopathy by increasing autophagy via the Act A/Smads signaling pathway.
Direct moxibustion exerts an analgesic effect on cervical spondylotic radiculopathy by increasing autophagy via the Act A/Smads signaling pathway.
复制标题
直接的moxibustion通过通过ACT A/SMADS信号通路来增加自噬，对颈脊柱辐射病具有镇痛作用。
DOI：
10.1002/brb3.2545
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发表时间：
2022-04
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
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作者：
直接灸治疗神经根型颈椎病已有报道，但其镇痛机制尚不清楚。自噬在神经元凋亡中起保护作用，Act A/Smads信号通路已被证实与自噬的激活有关。本研究旨在探讨DM对CSR大鼠自噬的影响及其与Act A/Smads信号通路的关系。将大鼠随机分为假手术组、CSR组、CSR + DM组、CSR + DM + 3-MA（PI 3 K抑制剂）组和CSR + DM + SB（Act A抑制剂）组。在神经根腋下插入鱼线建立CSR模型后3d，在大椎穴（GV 14）进行DM，6次，1次，连续7 d。Western blot和免疫荧光染色观察神经元自噬分子LC 3 II/I、Atg 7和Act A/Smads信号分子Act A、p-Smad 2和p-Smad 3的表达。采用真实的时间PCR检测Bcl-2/Bax mRNA表达。DM可改善CSR大鼠痛阈和运动功能，促进ActA、p-Smad 2、p-Smad 3、LC 3 II/I和Atg 7在神经根脊髓后角的表达。DM可降低Bax mRNA的表达，减少凋亡神经元的数量。3-MA和Act A抑制剂SB可抑制上述蛋白的表达，降低DM对凋亡神经元的保护作用。DM通过调节自噬减少细胞凋亡、修复神经损伤发挥镇痛作用，其机制可能与Act A/Smads信号通路有关。直接灸治疗神经根型颈椎病（CSR）大鼠模型后，痛阈和运动功能均得到改善。DM通过调节自噬减少CSR大鼠神经细胞凋亡，修复神经损伤，发挥镇痛作用。DM对自噬的调节作用可能是通过激活ActA/Smads通路实现的。
Direct moxibustion (DM) is reported to be useful for cervical spondylotic radiculopathy (CSR), but the analgesic mechanism remains unknown. Autophagy plays a protective role in neuronal apoptosis, Act A/Smads signaling pathway has been confirmed to be associated with the activation of autophagy. The study aimed to explore the effect of DM on autophagy in rats with CSR and the involvement of Act A/Smads signaling pathway. Rats were randomly divided into Sham, CSR, CSR + DM, CSR + DM + 3‐MA (PI3K inhibitor), and CSR + DM + SB (Act A inhibitor) group. Three days after establishment of CSR model with a fish line inserted under the axilla of the nerve roots, DM at Dazhui (GV14) was performed six times once for seven consecutive days. Western blot and immunofluorescence staining were used to observe the expression of the neuronal autophagy molecule LC3II/I, Atg7, and Act A/Smads signaling molecule Act A, p‐Smad2, and p‐Smad3. Bcl‐2/Bax mRNA expression was measured by real time PCR. DM improved the pain threshold and motor function of CSR rats and promoted the expression of Act A, p‐Smad2, p‐Smad3, LC3II/I, and Atg7 in the entrapped‐nerve root spinal dorsal horn. DM reduced the expression of Bax mRNA and decreased the number of apoptotic neurons. 3‐MA and Act A inhibitor SB suppressed the expression of above‐mentioned proteins and reduced the protective effect of DM on apoptotic neurons. DM exerts analgesic effects by regulating the autophagy to reduce cell apoptosis and repair nerve injury, and this feature may be related to the Act A/Smads signaling pathway. Pain threshold and motor function were improved after treatment with Direct Moxibustion (DM) in a rat model of cervical spondylotic radiculopathy (CSR). DM exerts analgesic effects by regulating the autophagy to reduce cell apoptosis and repair nerve injury in rats with CSR. The regulation effect of DM on autophagy may be achieved through activating the Act A/Smads pathway.