X-ray scattering for classifying tissue types associated with breast disease.
X-ray scattering for classifying tissue types associated with breast disease.
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DOI：
10.1118/1.2977667
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发表时间：
2008-10
期刊：
影响因子：
3.8
通讯作者：
S. Sidhu;K. Siu;G. Falzon;S. Nazaretian;S. Hart;J. Fox;B. Susil;R. Lewis
中科院分区：
文献类型：
--
作者：
S. Sidhu;K. Siu;G. Falzon;S. Nazaretian;S. Hart;J. Fox;B. Susil;R. Lewis
I型和III型胶原蛋白可使用小角X射线散射（SAXS）在分子水平（数十至数百纳米尺度）上表征。尽管在该长度尺度下的胶原原纤维结构参数已经显示出患病和未患病乳腺组织之间的差异，但是先前尚未研究涉及大（>50）患者队列的多个特征的综合分析。从80名患有乳房肿块或乳房缩小术的患者中切除乳房组织样本。从这些，浸润性癌，良性组织，和正常实质进行了分析。胶原蛋白结构相关的参数，包括纵向（轴向）和横向（赤道）的功能，极角功能，总散射强度，和组织异质性的影响，提取的SAXS模式和检查。三阶轴向峰的振幅和总散射强度（无定形散射）显示组织组之间的最大分离，并且使用这两个参数的分类模型在浸润性癌和乳房成形术患者之间表现出超过95%的准确性。取自无病患者（乳房成形术）的正常组织和取自存在疾病的患者的正常组织显示出显著差异，表明SAXS可提供与常规组织病理学不同的诊断信息。
Collagen types I and III can be characterized at the molecular level (at the tens to hundreds of nanometers scale) using small angle x-ray scattering (SAXS). Although collagen fibril structural parameters at this length scale have shown differences between diseased and nondiseased breast tissues, a comprehensive analysis involving a multitude of features with a large (>50) patient cohort has not previously been investigated. Breast tissue samples were excised from 80 patients presenting with either a breast lump or reduction mammoplasty. From these, invasive carcinoma, benign tissue, and normal parenchyma were analyzed. Parameters related to collagen structure, including longitudinal (axial) and lateral (equatorial) features, polar angle features, total scattering intensity, and tissue heterogeneity effects, were extracted from the SAXS patterns and examined. The amplitude of the third-order axial peak and the total scattering intensity (amorphous scatter) showed the most separation between tissue groups and a classification model using these two parameters demonstrated an accuracy of over 95% between invasive carcinoma and mammoplasty patients. Normal tissue taken from disease-free patients (mammoplasty) and normal tissue taken from patients with presence of disease showed significant differences, suggesting that SAXS may provide different diagnostic information from that of conventional histopathology.