Characterization of overexpression of the alternatively spliced isoform of the protein phosphatase 2A catalytic subunit in cells
Characterization of overexpression of the alternatively spliced isoform of the protein phosphatase 2A catalytic subunit in cells
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细胞中蛋白磷酸酶 2A 催化亚基的选择性剪接亚型过表达的表征。
DOI：
10.1016/j.bbrc.2017.10.093
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发表时间：
2017
影响因子：
3.1
通讯作者：
Xiyi Li
中科院分区：
文献类型：
--
作者：
Shen Tang;Yuyang Liu;Xinhang Wang;Ziwei Liang;Haiqing Cai;Laiming Mo;Deqiang Xiao;Songchao Guo;Yiqiang Ouyang;Bin Sun;Cailing Lu;Xiyi Li
PP 2 α 2是最近发现的一种PP 2Aca选择性剪接异构体，其可以在血清撤除后被诱导。它显示出增强的免疫球蛋白结合蛋白1的结合，overexpressed.in慢性淋巴细胞白血病患者。目前对PP 2 O2的了解有限。本研究从HL-60细胞和人淋巴细胞中诱导并克隆了PP 2 α 2，并将其转染人胚肾293细胞，建立了稳定过表达的细胞系。我们发现，PP 2 α 2 mRNA抑制其较长亚型PP 2Aca mRNA的表达，但对该较长亚型的最终蛋白表达和修饰没有影响。此外，PP 2 β 2过表达的细胞表现出IGBP 1表达增加，激活mTORC 1信号传导以减少基础自噬和增加锚定非依赖性生长。我们的研究为PP 2A调控的复杂机制提供了新的见解。
PP2Aca2 is a recently discovered PP2Aca alternative splicing isoform that can be induced following.serum withdrawal. It shows enhanced binding to immunoglobulin binding protein 1 and is overexpressed.in chronic lymphocytic leukemia patients. Current knowledge concerning PP2Aca2 is limited. In.this study, we induced and cloned PP2Aca2 from HL-60 cells and human lymphocytes, transfected them.into human embryonic kidney 293 cells and constructed a stable overexpression cell line. We found that.PP2Aca2 mRNA inhibits expression of its longer isoform PP2Aca mRNA but had no effect on the final.protein expression and modification of this longer isoform. Moreover, PP2Aca2-overexpressed cells.demonstrated increased expression of IGBP1, activated mTORC1 signaling to reduce basal autophagy and.increased anchorage-independent growth. Our study provides new insights into the complex mechanisms.of PP2A regulation.