STRONG IMMUNOREACTIVITY OF BETA-AMYLOID PRECURSOR PROTEIN, INCLUDING THE BETA-AMYLOID PROTEIN-SEQUENCE, AT HUMAN NEUROMUSCULAR-JUNCTIONS
STRONG IMMUNOREACTIVITY OF BETA-AMYLOID PRECURSOR PROTEIN, INCLUDING THE BETA-AMYLOID PROTEIN-SEQUENCE, AT HUMAN NEUROMUSCULAR-JUNCTIONS
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DOI：
10.1016/0304-3940(92)90241-x
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发表时间：
1992-08-31
影响因子：
2.5
通讯作者：
ALVAREZ, RB
中科院分区：
文献类型：
--
作者：
ASKANAS, V;ENGEL, WK;ALVAREZ, RB
At the postsynaptic domain of the human neuromuscular junction (NMJ), we have demonstrated strong concentrations of the N-terminus 45 62, C-terminus 676-695 and beta-amyloid protein sequences of beta-amyloid precursor protein (beta-APP). We used well-characterized monoclonal and polyclonal antibodies for co-localization with three other postsynaptic proteins, applying double and triple fluorescence labeling. Strong immunoreactivity of all three beta-APP sequences was found at all NMJs identified by bound alpha-bungarotoxin (alpha-BT), where they co-localized with alpha-BT and with immunoreactive desmin and dystrophin, which are postsynaptic proteins of human NMJs. This appears to be the first demonstration of beta-APP sequences concentrated postsynaptically at human NMJs. Beta-APP may have a role in normal junctional biology and possibly in some diseases affecting NMJs.
