Comparisons of the genetic structure of populations of Turnip mosaic virus in West and East Eurasia
Comparisons of the genetic structure of populations of Turnip mosaic virus in West and East Eurasia
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DOI：
10.1016/j.virol.2004.09.040
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发表时间：
2004-12-20
期刊：
影响因子：
3.7
通讯作者：
Ohshima, K
中科院分区：
文献类型：
--
作者：
Tomimura, K;Spak, J;Ohshima, K
通过对142个芜菁花叶病毒（Turnipmosaicwrits，TuMV）分离物的寄主范围和基因序列的比较，分析了欧亚大陆芜菁花叶病毒（Turnipmosaicwrits，TuMV）的遗传结构。大多数分离物收集在欧亚大陆西部感染芸苔属植物，而那些从欧亚大陆东部感染芸苔属和萝卜属植物。在基因组的五个区域（全序列的三分之一）的重组位点（RS）的分析表明，蛋白质1（P1基因）的重组频率比其他基因区域在两个亚群，但RS位于不同的部分亚群的基因组。核苷酸多样性的估计表明，西欧亚亚群比东欧亚亚群更多样化，但亚洲-BR组的基因，从后者亚群有一个更大的非同义/同义替换比，特别是在PI，病毒基因组连接蛋白（VPg）和核包涵体蛋白酶（NIa-Pro）基因。这些亚群似乎是独立于祖先欧洲种群进化而来的，它们的遗传结构可能反映了创始人效应。(C)2004爱思唯尔公司All rights reserved.
The genetic structure of populations of Turnip mosaic writs (TuMV) in Eurasia was assessed by making host range and gene sequence comparisons of 142 isolates. Most isolates collected in West Eurasia infected Brassica plants whereas those from East Eurasia infected both Brassica and Raphamus plants. Analyses of recombination sites (RSs) in five regions of the genome (one third of the full sequence) showed that the protein 1 (P1 gene) had recombined more frequently than the other gene regions in both subpopulations, but that the RSs were located in different parts of the genomes of the subpopulations. Estimates of nucleotide diversity showed that the West Eurasian subpopulation was more diverse than the East Eurasian subpopulation, but the Asian-BR group of the genes from the latter subpopulation had a greater nonsynonymous/synonymous substitution ratio, especially in the PI, viral genome-linked protein (VPg) and nuclear inclusion a proteinase (NIa-Pro) genes. These subpopulations seem to have evolved independently from the ancestral European population, and their genetic structure probably reflects founder effects. (C) 2004 Elsevier Inc. All rights reserved.