Early pregnancy diagnosis by means of ultrasonography as a method of improving reproductive efficiency in goats
Early pregnancy diagnosis by means of ultrasonography as a method of improving reproductive efficiency in goats
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DOI：
10.1262/jrd.50.391
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发表时间：
2004-08-01
影响因子：
1.8
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Medan, M;Watanabe, G;Taya, K
本研究选用18只周期性柴犬。在B超检查至少一个黄体后，单次注射125 μ g合成的前列腺素F-2 α（PGF（2）α）类似物，使发情同步。在交配后第0、7和21天从每只动物采集血样用于孕酮测定。发情期的动物在发情期间被允许与能育的巴克斯交配两次（组I; n=12）或根本不交配（组II; n=6）。使用配备7.5或5 MHz探头的实时B型扫描仪经直肠或经腹部进行超声检查。所有动物在注射PGF 2 α后56.0 +/- 2.7 h表现出发情。结果表明，孕酮测定在交配后第21天诊断妊娠的准确性为80%，回顾性地为100%。超声检查显示，孕囊和胚胎心跳分别在妊娠第20.2 +/- 0.6和24.3 +/- 0.7天检测到。在妊娠第35.4 +/- 1.0天检测到小结节状胎盘（大小为0.7 +/- 0.2 cm）。在怀孕两个月时，骨骼结构，如头骨，胸部和长骨清晰。颅骨双顶径和长骨长度可用作胎龄的估计值。实时B型超声在孕60天检测胎儿个数的准确率为91.7%。因此，在交配后第21天测定孕酮（临界值，1 ng/ml）可用于山羊妊娠诊断。然而，B型经直肠超声检查是更有效的，由于检测胚胎和确认其活力的心跳。此外，胎儿数量和胎龄只能通过超声检查确定。
Eighteen cyclic Shiba goats were used in this study. Estrus was synchronized with a single injection of 125 mu g of a synthetic analogue of prostaglandin F-2 alpha (PGF(2)alpha) after detection of at least one corpus luteum by B-mode ultrasonography. Blood samples were collected from each animal on days 0, 7 and 21 post-mating for progesterone assay. Animals in estrus were either allowed to be mated by fertile bucks twice during estrus (group I; n=12) or not at all (group II; n=6). Ultrasonographic examinations were performed transrectally or transabdominally using a real-time B-mode scanner equipped with a 7.5 or 5 MHz transducer. All animals exhibited estrus 56.0 +/- 2.7 h after injection of PGF2 alpha. The results show that the accuracy of the progesterone assay in diagnosing pregnancy on day 21 after mating was 80% for pregnancy and 100% for non-pregnancy, retrospectively. Ultrasonographic examinations showed that gestational sac and embryos heartbeats were detected on days 20.2 +/- 0.6 and 24.3 +/- 0.7 of gestation, respectively. Placentomes were detected on day 35.4 +/- 1.0 of gestation as small nodules (0.7 +/- 0.2 cm in size). At two months pregnancy, skeletal structures like skull, thorax and long bones were clear. Biparietal diameter of the skull and length of long bones could be used as an estimate of gestational age. The accuracy of detection of fetal number using real-time B-mode ultrasonography was 91.7% on day 60 of gestation. In conclusion, progesterone assay at day 21 post-mating (cut-off value, 1 ng/ml) can be used for pregnancy diagnosis in goats. However, B-mode transrectal ultrasonography was more efficient due to detection of embryo and confirmation of its viability by heartbeats. In addition, fetal number and gestational age could be determined only by ultrasonography.