Arabidopsis thaliana constitutively active ROP11 interacts with the NADPH oxidase respiratory burst oxidase homologue F to regulate reactive oxygen species production in root hairs
Arabidopsis thaliana constitutively active ROP11 interacts with the NADPH oxidase respiratory burst oxidase homologue F to regulate reactive oxygen species production in root hairs
复制标题
拟南芥组成型活性ROP11与NADPH氧化酶呼吸爆发氧化酶同源物F相互作用调节根毛中活性氧的产生
DOI：
10.1071/fp15090
复制
发表时间：
2016
影响因子：
3
通讯作者：
Zhang WH
中科院分区：
文献类型：
--
作者：
Yan Min;Jing Wen;Xu Ni;Shen Like;Zhang Qun;Zhang Wenhua;Zhang Q;Zhang WH
活性氧(ROS)在细胞内起着关键的信号转导作用。植物NADPH氧化酶，也被称为呼吸爆发氧化酶同系物(Rbohs)，是具有良好特征的ROS产生系统。在这项研究中，我们通过酵母双杂交分析、下拉实验和体内双分子荧光互补实验发现，具有结构性活性的小鸟苷三磷酸酶(GTPase)ROP11(CA-ROP11)与RbohF相互作用。RbohF中氨基酸L336或L337的突变使其与CA-ROP11的相互作用消失。与CA-ROP11和突变体RbohF或显性负值ROP11和野生型RbohF共表达相比，CA-ROP11和野生型RbohF共表达能促进烟草体内ROS的产生。此外，CA-ROP11过表达导致ROS的积累和拟南芥根毛的肿胀表型。海因。RbohF基因的缺失部分降低了高表达CA-ROP11的拟南芥植株中ROS的增加。这些结果表明，拟南芥ROP11通过与根毛中的RbohF相互作用来调节ROS的产生。
Reactive oxygen species (ROS) play a key signalling role in cells. Plant NADPH oxidases, also known as respiratory burst oxidase homologues (Rbohs), are well characterised ROS-generating systems. In this study, we found that the constitutively active small guanosine triphosphatase (GTPase) ROP11 (CA-ROP11) interacted with RbohF by using a yeast two-hybrid analysis, a pull-down assay and an in vivo bimolecular fluorescence complementation assay. The mutation of amino acid L336 or L337 in RbohF abolished its interaction with CA-ROP11. Coexpression of CA-ROP11 and wild-type RbohF in Nicotiana benthamiana Domin enhanced ROS production compared with coexpression of CA-ROP11 and mutant RbohF or of dominant negative ROP11 and wild-type RbohF. Moreover, CA-ROP11 overexpression resulted in ROS accumulation and a swollen root hair phenotype in Arabidopsis thaliana (L.) Heynh. The deletion of RbohF partially reduced the increase in ROS in Arabidopsis plants overexpressing CA-ROP11. These results suggest that Arabidopsis ROP11 modulates ROS production by interacting with RbohF in root hairs.