New stent surface materials: The impact of polymer-dependent interactions of human endothelial cells, smooth muscle cells, and platelets
New stent surface materials: The impact of polymer-dependent interactions of human endothelial cells, smooth muscle cells, and platelets
复制标题
DOI：
10.1016/j.actbio.2013.10.015
复制
发表时间：
2014-02-01
影响因子：
9.7
通讯作者：
Sternberg, Katrin
中科院分区：
文献类型：
--
作者：
Busch, Raila;Strohbach, Anne;Sternberg, Katrin
尽管新型冠状动脉支架技术不断发展，但支架内再狭窄和支架内血栓形成仍然具有临床意义。血液和组织细胞与植入材料的相互作用可能是这些副作用的重要原因。我们假设血液和组织细胞的材料依赖性相互作用。因此，本研究的目的是研究血管内皮细胞、平滑肌细胞和血小板与各种可生物降解聚合物的影响，以确定具有优异内皮细胞支持和非血栓形成特性的支架涂层或平台材料。将人脐静脉内皮细胞、人冠状动脉内皮细胞和人冠状动脉平滑肌细胞培养在两种用于药物洗脱支架的生物稳定聚合物，即聚（乙烯-醋酸乙烯酯）（PEVA）和聚（甲基丙烯酸丁酯）（PBMA）的表面上。我们将这些聚合物与新型可生物降解聚酯聚丙交酯 (PLLA)、聚 3-羟基丁酸酯 (P(3HB))、聚 4-羟基丁酸酯 (P(4HB)) 以及 PLLA/P(4HB) 比例为 78/22% (w/w) 的聚合物共混物进行了比较。在静态和动态条件下进行生物相容性测试。细胞增殖、活力、糖被宽度、eNOS 和 PECAM-1 mRNA 表达的测量揭示了所研究的六种聚合物样品之间强烈的材料依赖性。只有 PLLA/P(4HB) 聚合物混合物才能实现优异的生物相容性内皮标志物。数据显示，PLLA 和 P(4HB) 更容易产生血栓反应，而聚合物共混物的特点是血栓形成可能性较低。这些数据证明了材料依赖性内皮化、平滑肌细胞生长和血栓形成性。尽管 PEVA 和 PBMA 等聚合物已普遍用于血管植入物，但它们并未充分满足生物相容性标准。所研究的可生物降解聚合物混合物 PLLA/P(4HB) 显然代表了一种有前途的血管支架和支架涂层材料。 (C) 2013 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Despite the development of new coronary stent technologies, in-stent restenosis and stent thrombosis are still clinically relevant. Interactions of blood and tissue cells with the implanted material may represent an important cause of these side effects. We hypothesize material-dependent interaction of blood and tissue cells. The aim of this study is accordingly to investigate the impact of vascular endothelial cells, smooth muscle cells and platelets with various biodegradable polymers to identify a stent coating or platform material that demonstrates excellent endothelial-cell-supportive and non-thrombogenic properties. Human umbilical venous endothelial cells, human coronary arterial endothelial cells and human coronary arterial smooth muscle cells were cultivated on the surfaces of two established biostable polymers used for drug-eluting stents, namely poly(ethylene-co-vinylacetate) (PEVA) and poly(butyl methacrylate) (PBMA). We compared these polymers to new biodegradable polyesters poly(c-lactide) (PLLA), poly(3-hydroxybutyrate) (P(3HB)), poly(4-hydroxybutyrate) (P(4HB)) and a polymeric blend of PLLA/P(4HB) in a ratio of 78/22% (w/w). Biocompatibility tests were performed under static and dynamic conditions. Measurement of cell proliferation, viability, glycocalix width, eNOS and PECAM-1 mRNA expression revealed strong material dependency among the six polymer samples investigated. Only the polymeric blend of PLLA/P(4HB) achieved excellent endothelial markers of biocompatibility. Data show that PLLA and P(4HB) tend to a more thrombotic response, whereas the polymer blend is characterized by a lower thrombotic potential. These data demonstrate material-dependent endothelialization, smooth muscle cell growth and thrombogenicity. Although polymers such as PEVA and PBMA are already commonly used for vascular implants, they did not sufficiently meet the criteria for biocompatibility. The investigated biodegradable polymeric blend PLLA/P(4HB) evidently represents a promising material for vascular stents and stent coatings. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.