Nano-flares: Probes for transfection and mRNA detection in living cells
Nano-flares: Probes for transfection and mRNA detection in living cells
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DOI：
10.1021/ja0776529
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发表时间：
2007-12-19
影响因子：
15
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Seferos, Dwight S.;Giljohann, David A.;Mirkin, Chad A.
我们证明了新型的寡核苷酸修饰的金纳米粒子探针与荧光团标记的补体杂交，既可以用作转染剂，也可以用作细胞“纳米耀斑”，用于检测活细胞中的mRNA。纳米火炬利用了金的高效荧光猝灭特性，寡核苷酸纳米颗粒偶联物的细胞摄取而不使用转染剂，以及这种偶联物的酶促稳定性，从而克服了创造敏感和有效的细胞内探针的许多挑战，纳米火炬表现出高信号，具有低背景荧光，并且对细胞中存在的RNA转录物数量的变化敏感。
We demonstrate that novel oligonucleotide-modified gold nanoparticle probes hybridized to fluorophore-labeled complements can be used as both transfection agents and cellular "nano-flares" for detecting mRNA in living cells. Nano-flares take advantage of the highly efficient fluorescence quenching properties of gold, cellular uptake of oligonucleotide nanoparticle conjugates without the use of transfection agents, and the enzymatic stability of such conjugates, thus overcoming many of the challenges to creating sensitive and effective intracellular probes, Nano-flares exhibit high signaling, have low background fluorescence, and are sensitive to changes in the number of RNA transcripts present in cells.