Live imaging of single nuclear pores reveals unique assembly kinetics and mechanism in interphase
Live imaging of single nuclear pores reveals unique assembly kinetics and mechanism in interphase
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DOI：
10.1083/jcb.201007076
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发表时间：
2010-10-04
影响因子：
7.8
通讯作者：
Ellenberg, Jan
中科院分区：
文献类型：
--
作者：
Dultz, Elisa;Ellenberg, Jan
在后生动物中，新的核孔复合物（NPC）在两个不同的细胞周期阶段形成：在有丝分裂结束时伴随着核膜的重新形成和在间期。然而，这些组装过程的机制可能不同。在这项研究中，我们应用高分辨率活细胞显微镜来分析单个NPC在活哺乳动物细胞间期的动态。我们发现，核生长和NPC大会是相关的，并发生在一个恒定的速率在整个间期。通过分析单个NPC组装事件的动力学，我们证明它们是由膜核孔蛋白Pom 121的缓慢积累引发的，随后是可溶性NPC亚复合物Nup 107 -160的更快速的缔合。与有丝分裂后NPC组装相比，这种相反的招募顺序和整体慢得多的动力学支持这两个过程通过不同的分子机制发生的结论。
In metazoa, new nuclear pore complexes (NPCs) form at two different cell cycle stages: at the end of mitosis concomitant with the reformation of the nuclear envelope and during interphase. However, the mechanisms of these assembly processes may differ. In this study, we apply high resolution live cell microscopy to analyze the dynamics of single NPCs in living mammalian cells during interphase. We show that nuclear growth and NPC assembly are correlated and occur at a constant rate throughout interphase. By analyzing the kinetics of individual NPC assembly events, we demonstrate that they are initiated by slow accumulation of the membrane nucleoporin Pom121 followed by the more rapid association of the soluble NPC subcomplex Nup107-160. This inverse order of recruitment and the overall much slower kinetics compared with postmitotic NPC assembly support the conclusion that the two processes occur by distinct molecular mechanisms.