Molecular typing methods for Pasteurella multocida-A review
Molecular typing methods for Pasteurella multocida-A review
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DOI：
10.13343/j.cnki.wsxb.20160002
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发表时间：
2016-10-01
期刊：
影响因子：
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通讯作者：
Wu, Bin
中科院分区：
文献类型：
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作者：
Peng, Zhong;Liang, Wan;Wu, Bin
多杀性巴氏杆菌是一种能感染多种动物甚至是人的重要革兰氏阴性病原菌。人类也可能通过动物咬伤或抓挠而感染多杀性巴氏杆菌。目前临床上用于多杀性巴氏杆菌诊断的分型方法主要包括血清学分型方法和分子分型方法。其中，血清学分型方法以免疫学测定为基础，对于临床细菌学研究来说过于复杂。而多重PCR和多位点序列分型（MLST）等分子生物学方法更适合于临床多杀性巴氏杆菌的细菌学研究，与传统的血清学分型方法相比，具有操作简单、效率高、检测准确等优点，因此得到了广泛应用。本文就多杀性巴氏杆菌的分子分型方法作一简要综述。为临床细菌学研究，特别是多杀性巴氏杆菌的分子生物学研究提供基础知识。
Pasteurella multocida is an important gram-negative pathogenic bacterium that could infect wide ranges of animals. Humans could also be infected by P. multocida via animal bite or scratching. Current typing methods for P. multocida include serological typing methods and molecular typing methods. Of them, serological typing methods are based on immunological assays, which are too complicated for clinical bacteriological studies. However, the molecular methods including multiple PCRs and multilocus sequence typing (MLST) methods are more suitable for bacteriological studies of P. multocida in clinic, with their simple operation, high efficiency and accurate detection compared to the traditional serological typing methods, they are therefore widely used. In the current review, we briefly describe the molecular typing methods for P. multocida. Our aim is to provide a knowledge-foundation for clinical bacteriological investigation especially the molecular investigation for P. multocida.