The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence
The importance of mitochondrial metabolic activity and mitochondrial DNA replication during oocyte maturation in vitro on oocyte quality and subsequent embryo developmental competence
复制标题
卵母细胞体外成熟过程中线粒体代谢活动和线粒体 DNA 复制对卵母细胞质量和随后胚胎发育能力的重要性
DOI：
10.1002/mrd.22042
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发表时间：
2012-06-01
影响因子：
2.5
通讯作者：
Dong, Qiaoxiang
中科院分区：
文献类型：
--
作者：
Ge, Hongshan;Tollner, Theodore L.;Dong, Qiaoxiang
线粒体代谢能力和DNA复制都被证明会影响卵母细胞的质量，但尚不清楚哪一个更重要。在本研究中，未成熟卵母细胞分别用FCCP或ddC处理，分别抑制卵母细胞体外成熟过程中各自的线粒体代谢能力或DNA复制。为了区分它们的作用，我们评估了与卵母细胞成熟（胚泡破裂和核成熟）、质量（纺锤体形成、染色体排列和线粒体分布模式）、受精能力和随后的胚胎发育能力（囊胚形成和囊胚细胞数量）相关的各种参数。FCCP抑制线粒体代谢能力导致核成熟卵母细胞百分比降低；正常纺锤体形成和染色体排列；线粒体分布均匀；以及形成囊胚的能力。ddC抑制mtDNA复制对卵母细胞成熟和线粒体分布没有明显影响，尽管高剂量ddC增加了卵母细胞出现异常纺锤体形成和染色体排列的百分比。然而，ddC确实能显著减少囊胚的形成。FCCP和ddC对受精率均无影响。这些发现表明，与较低的线粒体DNA拷贝数相关的影响与卵母细胞中线粒体代谢活性降低的影响并不一致。在卵母细胞成熟过程中抑制线粒体代谢活性对卵母细胞成熟和随后的胚胎发育能力有负面影响。另一方面，线粒体DNA拷贝数的减少主要影响胚胎发育潜力，但对卵母细胞成熟和体外受精的影响不大。摩尔。天线转换开关。Dev. 79:392 - 401,2012。©2012 Wiley期刊公司
Mitochondrial metabolic capacity and DNA replication have both been shown to affect oocyte quality, but it is unclear which one is more critical. In this study, immature oocytes were treated with FCCP or ddC to independently inhibit the respective mitochondrial metabolic capacity or DNA replication of oocytes during in vitro maturation. To differentiate their roles, we evaluated various parameters related to oocyte maturation (germinal vesicle break down and nuclear maturation), quality (spindle formation, chromosome alignment, and mitochondrial distribution pattern), fertilization capability, and subsequent embryo developmental competence (blastocyst formation and cell number of blastocyst). Inhibition of mitochondrial metabolic capacity with FCCP resulted in a reduced percent of oocytes with nuclear maturation; normal spindle formation and chromosome alignment; evenly distributed mitochondria; and an ability to form blastocysts. Inhibition of mtDNA replication with ddC has no detectable effect on oocyte maturation and mitochondrial distribution, although high‐dose ddC increased the percent of oocytes showing abnormal spindle formation and chromosome alignment. ddC did, however, reduce blastocyst formation significantly. Neither FCCP nor ddC exposure had an effect on the rate of fertilization. These findings suggest that the effects associated with lower mitochondrial DNA copy number do not coincide with the effects seen with reduced mitochondrial metabolic activity in oocytes. Inhibiting mitochondrial metabolic activity during oocyte maturation has a negative impact on oocyte maturation and subsequent embryo developmental competence. A reduction in mitochondrial DNA copy number, on the other hand, mainly affects embryonic development potential, but has little effect on oocyte maturation and in vitro fertilization. Mol. Reprod. Dev. 79:392–401, 2012. © 2012 Wiley Periodicals, Inc.