The heterochronic geneOryza sativa LIKE HETEROCHROMATIN PROTEIN 1modulates miR156b/c/i/e levels
The heterochronic geneOryza sativa LIKE HETEROCHROMATIN PROTEIN 1modulates miR156b/c/i/e levels
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异时性基因Oryza sativa LIKE HETEROCHROMATIN PROTEIN 1调节miR156b/c/i/e水平
DOI：
10.1111/jipb.12991
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发表时间：
2020-08-12
影响因子：
11.4
通讯作者：
Hu, Xingming
中科院分区：
文献类型：
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作者：
Cui, Yongtao;Cheng, Jingfei;Hu, Xingming
植物从幼年到成年的转变涉及营养生长和植物结构的变化；这一转变的时机对农业具有重要影响。 microRNA miR156 调节植物中的这种转变和芽成熟。在拟南芥中，MIR156A/Cloci 的赖氨酸 27（H3K27me3，抑制标记）上的组蛋白 H3 三甲基化沉积受到 Polycomb 抑制复合物 1 (PRC1) 或 PRC2 的调节，具体取决于发育阶段。 miR156 的水平在芽成熟过程中逐渐下降。 MIR156A/Cloci 处的 H3K27me3 量影响 miR156 水平；然而，这种表观遗传调控是否保守仍不清楚。在这里，我们发现在水稻（Oryza sativa）中，假定的 PRC1 亚基如异染色质蛋白 1 (OsLHP1) 与 miR156-SQUAMOSA 启动子结合蛋白样 (SPL) 模块会影响发育阶段的转变。 OsLHP1 功能的丧失导致 MIR156B/C/I/E 的异位表达、miR156 过表达的表型以及 MIR156B/C/I/E 的 H3k27me3 水平降低。这表明OsLHP1在功能上与拟南芥LHP1不同。对野生型、miR156b/c 过表达和 Oslhp1-2 突变植物的遗传和转录组分析表明，OsLHP1 在幼年到成年的转变过程中在 miR156 和 SPL 的上游发挥作用。因此，修改 OsLHP1-miR156-SPL 途径可能能够改变营养期和植物结构。
The juvenile-to-adult transition in plants involves changes in vegetative growth and plant architecture; the timing of this transition has important implications for agriculture. The microRNA miR156 regulates this transition and shoot maturation in plants. InArabidopsis thaliana, deposition of histone H3 trimethylation on lysine 27 (H3K27me3, a repressive mark) at theMIR156A/Cloci is regulated by Polycomb Repressive Complex 1 (PRC1) or PRC2, depending on the developmental stage. The levels of miR156 progressively decline during shoot maturation. The amount of H3K27me3 atMIR156A/Cloci affects miR156 levels; however, whether this epigenetic regulation is conserved remains unclear. Here, we found that in rice (Oryza sativa), the putative PRC1 subunit LIKE HETEROCHROMATIN PROTEIN 1 (OsLHP1), with the miR156-SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) module, affects developmental phase transitions. Loss of OsLHP1 function results in ectopic expression ofMIR156B/C/I/E, phenocopy of miR156 overexpression, and reduced H3k27me3 levels atMIR156B/C/I/E. This indicates thatOsLHP1has functionally diverged fromArabidopsis LHP1. Genetic and transcriptome analyses of wild-type, miR156b/c-overexpression, andOslhp1-2mutant plants suggest that OsLHP1 acts upstream of miR156 and SPL during the juvenile-to-adult transition. Therefore, modifying the OsLHP1-miR156-SPL pathway may enable alteration of the vegetative period and plant architecture.