A generic and label free method based on dielectrophoresis for the continuous separation of microorganism from whole blood samples
A generic and label free method based on dielectrophoresis for the continuous separation of microorganism from whole blood samples
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DOI：
10.1016/j.snb.2015.02.024
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发表时间：
2015-06-01
影响因子：
8.4
通讯作者：
Le Pioufle, Bruno
中科院分区：
文献类型：
--
作者：
Bisceglia, Emile;Cubizolles, Myriam;Le Pioufle, Bruno
目前用于血流感染的体外诊断的方法是基于血液培养，随后是病原体的表型表征。然而，在需要快速和适当的医学治疗的情况下，这种方法太长了。因此，人们对与现有分析手段结合的血液样品解络合方法非常感兴趣。在本文中，我们报告了连续流捕获和浓度的血液样品中掺入的微生物，在一个专用的微流体装置内流动。使用缓冲液的低渗条件，通过介电泳（DEP）力实现分离。我们证明了在我们的微流体装置中从血液中有效和实时地积累目标病原体，从而提供了适合进一步表征的去复杂化样品。特别是，在大肠杆菌微生物中可以观察到高达97%的捕获率。还可以实现不同微生物（革兰氏阳性菌、革兰氏阴性菌和酵母菌）的同时分离，显示了该方法和装置的多功能性。(C)2015 Elsevier B.V.版权所有。
Current methods for the in vitro diagnostics of bloodstream infections are based on blood culture, followed by phenotypic characterization of the pathogen. Nevertheless, such methods are too long in the case where a fast and appropriate medical treatment is needed. There is thus a very strong interest in blood sample decomplexification methods to be coupled to the existing analytical means. In this paper, we report the continuous flow capture and concentration of microorganisms spiked in a blood sample, flowing within a dedicated microfluidic device. The separation is achieved with dielectrophoresis (DEP) forces, using hypotonic conditions for the buffer. We demonstrate efficient and real-time accumulation of target pathogens from blood in our microfluidic device, thus providing a decomplexified sample suitable for further characterization. In particular, up to 97% capture rate could be observed with Escherichia coli micro-organisms. Simultaneous separation of different micro-organisms (Gram+, Gram- and yeast) could also be achieved, showing the versatility of the method and device. (C) 2015 Elsevier B.V. All rights reserved.