Protective effect of ellagic acid on healing alveolar bone after tooth extraction in rat-A histological and immunohistochemical study
Protective effect of ellagic acid on healing alveolar bone after tooth extraction in rat-A histological and immunohistochemical study
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DOI：
10.1016/j.archoralbio.2014.06.001
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发表时间：
2014-09-01
影响因子：
3
通讯作者：
Rouhollahi, Elham
中科院分区：
文献类型：
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作者：
Al-Obaidi, Mazen M. Jamil;Al-Bayaty, Fouad Hussain;Rouhollahi, Elham
目的：本研究试图评估鞣花酸（EA）对大鼠拔牙后牙槽骨愈合的影响。设计：选择24只Sprague道利（SD）雄性大鼠（200-250 g），麻醉后拔除左上切牙。然后，将大鼠分为两组，每组12只;第一组被认为是对照组，仅给予生理盐水，而第二组（治疗组）每天灌胃给予EA，共28天。然后在第7、14、21和28天从每组中选择3只大鼠，解剖其上颌骨组织用于组织学观察和均质化目的。将组织固定、脱钙并包埋在石蜡中。制备5 μ m厚的连续切片并用苏木精和伊红（H&E）染色用于组织学研究。取类似切片进行免疫组织化学分析，以评估骨钙素（OSC）和骨桥蛋白（OPN）。此外，丙二醛（MDA）和超氧化物歧化酶（SOD）测定匀浆牙龈上颌骨组织的大鼠通过商业试剂盒。结果：根据组织学分析，我们已经确定，EA治疗诱导早期骨小梁沉积的治疗组，导致更有组织的骨基质在拔牙后的第14，21和28天，与对照组。与对照组相比，治疗组的OSC和OPN的阳性标记在第14天和第21天在牙槽窝中高度表达，这表明在拔牙后矿化过程开始时可能形成新的骨小梁。电针治疗组脂质过氧化（MDA）水平明显低于对照组（P < 0.05）。而电针组上颌骨组织中抗氧化防御酶（SOD）明显升高提示电针对拔牙后大鼠脂质过氧化损伤具有保护作用，尤其是电针治疗28 d后，其保护作用更为明显。本研究表明，电针可促进大鼠牙槽骨的愈合。此外，与对照组相比，电针治疗组显示出更强的阳性免疫标记的OSC和OPN。(C)2014爱思唯尔有限公司版权所有。
Objective: This study has attempted to evaluate the effects of ellagic acid (EA) on alveolar bone healing after tooth extraction in rats.Design: Twenty-four Sprague Dawley (SD) male rats (200-250 g) were selected and were anaesthetised for the extraction of upper left incisor. Then, the rats were divided into two groups, comprising 12 rats each; the first group has been considered as a control group and was given only normal saline, whereas, the second group (treated group) was intragastrically administrated with EA daily once, for 28 days. Then three rats from each group had been selected on 7th, 14th, 21st, and 28th days to dissect their maxilla tissue either for histological observation and homogenisation purposes. The tissues fixed, decalcified and embedded in paraffin. Serial sections of 5 mu m thickness were prepared and stained with haematoxylin and eosin (H&E) for the histological study. Similar sections were taken for immunohistochemical analysis to assess osteocalcin (OSC) and osteopontin (OPN). Furthermore, Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured in homogenated gingival maxilla tissue of rat by commercial kit.Results: Based on the histological analysis we have identified that, EA treatment has induced earlier trabecular bone deposition in the treated group, resulting in more organised bone matrix on the 14th, 21st, and 28th days after tooth extraction, as against the control group. In comparison to control group, the positive labelling of OSC and OPN of the treated group have been highly expressed in the alveolar socket on 14th, and 21st days, which has indicated a the possibility of formation of new bone trabeculae at the beginning of the mineralisation process, after tooth extraction. In the EA treatment group, lipid per-oxidation (MDA) was significantly decreased (P < 0.05), as opposed to the control group. However, the antioxidant defense enzyme (SOD) was significantly increased in the maxilla tissue treated with EA (P < 0.05), compared to control group, which suggests that, after tooth extraction, EA plays an important role in the protection against the induction of lipid per-oxidation, particularly after 28 days of treatment with EA.Conclusion: This study has concluded that, EA may accelerated the healing process in teeth socket of rats. Furthermore, the EA treated group showed a stronger positive immunolabelling for OSC and OPN, when compared with the control group. (C) 2014 Elsevier Ltd. All rights reserved.