A simultaneous optical and electricalin-vitroneuronal recording system to evaluate microelectrode performance
A simultaneous optical and electricalin-vitroneuronal recording system to evaluate microelectrode performance
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DOI：
10.1371/journal.pone.0237709
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发表时间：
2020-08-20
期刊：
影响因子：
3.7
通讯作者：
Svirskis, Darren
中科院分区：
文献类型：
--
作者：
Aqrawe, Zaid;Patel, Nitish;Svirskis, Darren
目标在本文中，我们的目的是详细的设置高时空分辨率，电记录系统，利用平面微电极阵列与同时光学成像适合于评估微电极性能与建议的“性能因子”度量。方法将有助于低噪声电记录的技术与电压敏感染料相结合，通过定制的放大系统和光学通过高速CMOS相机记录神经元活动。通过传统的信噪比（SNR）计算以及提出的性能因子，将该技术应用于金和聚（3，4-乙撑二氧噻吩）/聚苯乙烯磺酸盐（PEDOT/PSS）涂层电极的微电极记录性能。结果电、光技术同时记录神经元活动，应用河豚毒素抑制动作电位放电证实了神经元活动。使用两种测量，PEDOT/PSS优于金，然而，性能因子度量估计与金相比时信号转导改善3倍，而SNR估计与金相比时改善8倍。结论成功实现了一种通过微电极阵列进行电记录和通过电压敏感染料进行光学记录的系统的设计和功能。意义电和光学方法的高时空分辨率将允许一系列的应用，如改进的阈下突触事件的检测，尖峰分选算法的验证和提供了一个强大的评价细胞外微电极的性能。
Objectives In this paper, we aim to detail the setup of a high spatio-temporal resolution, electrical recording system utilising planar microelectrode arrays with simultaneous optical imaging suitable for evaluating microelectrode performance with a proposed ' performance factor ' metric. Methods Techniques that would facilitate low noise electrical recordings were coupled with voltage sensitive dyes and neuronal activity was recorded both electrically via a customised amplification system and optically via a high speed CMOS camera. This technique was applied to characterise microelectrode recording performance of gold and poly(3,4-ethylenedioxythiophene)/polystyrene sulfonate (PEDOT/PSS) coated electrodes through traditional signal to noise (SNR) calculations as well as the proposed performance factor. Results Neuronal activity was simultaneously recorded using both electrical and optical techniques and this activity was confirmed via tetrodotoxin application to inhibit action potential firing. PEDOT/PSS outperformed gold using both measurements, however, the performance factor metric estimated a 3 fold improvement in signal transduction when compared to gold, whereas SNR estimated an 8 fold improvement when compared to gold. Conclusion The design and functionality of a system to record from neurons both electrically, through microelectrode arrays, and optically via voltage sensitive dyes was successfully achieved. Significance The high spatiotemporal resolution of both electrical and optical methods will allow for an array of applications such as improved detection of subthreshold synaptic events, validation of spike sorting algorithms and a provides a robust evaluation of extracellular microelectrode performance.