Proliferative heterogeneity of vascular smooth muscle cells and its alteration by injury.
Proliferative heterogeneity of vascular smooth muscle cells and its alteration by injury.
复制标题
血管平滑肌细胞的增殖异质性及其损伤引起的改变。
DOI：
10.1016/0014-4827(85)90121-1
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Grunwald,J
中科院分区：
文献类型：
--
作者：
Haudenschild,CC;Grunwald,J
本研究旨在探讨血管壁中形态相似的平滑肌细胞(SMC)群体在功能上是同质的还是异质的，这与它们对损伤的增殖反应有关。我们使用延时录像测量了原始SMC克隆、兄弟姐妹对和母女对的分裂间时间(IDT)。来自体内未受干扰血管的SMC在IDT中表现出克隆间和克隆内的异质性，范围很广。体内球囊损伤导致IDT均匀缩短。损伤血管内皮细胞的IDT有80%在14h以内，而正常血管内皮细胞的IDT只有一半以上。对母子对的纵向分析证实，在未受干扰的血管壁中存在不同种类的SMC群体。在体内球囊损伤不仅缩短了大多数细胞的IDT，而且更短的IDT持续时间比未受干扰的血管长得多。我们认为，根据SMC对损伤的增殖反应，现在可以将主动脉中形态均一的SMC群体细分为几组功能不同的SMC。
This study was designed to explore the question of whether the population of morphologically similar smooth muscle cells (SMC) in the vessel wall is functionally homogeneous or heterogeneous with respect to their proliferative response to injury. Using time-lapse video recording we measured interdivision times (IDT) of primary SMC clones, sibling pairs, and mother/daughter pairs. SMC from in vivo undisturbed vessels displayed an interclonal and intraclonal heterogeneity with a wide range in IDT. In vivo balloon injury resulted in a population with homogeneously short IDT. While 80% of IDT of SMC from injured vessels were shorter than 14 h, only slightly more than half of IDT of cells from undisturbed vessels fell into this category. Longitudinal analysis of mother/daughter pairs confirmed the presence of a heterogeneous population of SMC in the undisturbed vessel wall. In vivo balloon injury not only shortened the IDT of the majority of cells, but the shorter IDT persisted much longer than in the case of the undisturbed vessel. We suggest that a morphologically homogeneous SMC population in the aorta can now be subdivided into several groups of functionally different SMC with respect to their proliferative response to injury.